【摘要】 目的　研究T淋巴细胞亚群在复发性单纯疱疹病毒性基质性角膜炎 (stromalkeratitis,SK)中的作用。方法　采用复发性单纯疱疹病毒性SK的NIH鼠模型 ,应用单克隆抗体GK 1 5、H 35选择性地排除鼠体内的CD+ 4、CD+ 8T淋巴细胞后 ,观察鼠的SK的复发率、临床特征、角膜病理切片及鼠的迟发型超敏反应 (delayed hypersensitivity ,DTH)的能力。结果　 90 %以上的CD+ 4T淋巴细胞及95 %以上的CD+ 8T淋巴细胞被排除。CD+ 4T淋巴细胞的排除降低了SK的复发率 ,减轻了SK的疾病程度 ,损伤了鼠的DTH反应能力。而CD+ 8T淋巴细胞的排除对此却无明显影响。SK的角膜病理显示 :排除了CD+ 8T淋巴细胞的鼠的角膜基质层内可见大量的淋巴细胞和中性粒细胞浸润 (类似于对照组角膜病理改变 ) ,而排除了CD+ 4T淋巴细胞的鼠的角膜基质层内偶见淋巴细胞浸润。结论　CD+ 4T淋巴细胞是介导复发性单纯疱疹病毒性SK免疫病理反应的主要因子。更多还原

【Abstract】 Objective To investigate the role of T lymphocyte subsets in the recurrent herpetic stromal keratitis (SK). Methods In a recurrent SK NIH mouse model, rat monoclonal antibodies were used to selectively deplete in vivo the CD + 4 and CD + 8 T lymphocytes in mice, and the effects of the subsets on recurrent herpetic SK were evaluated. Results As measured by flow cytometry, in the mice treated with GK1.5, >90% of CD + 4 T cells were depleted; and in the mice treated with H 35, >95% of CD + 8 T cells were depleted. Depletion of CD + 4 T cells could halt the recurrence of SK, reduce the severity of recurrent SK and impair the delayed type hypersensitivity. In contrast, depletion of CD + 8 T cells had no effect on the outcome of the disease. Histopathologic examination of the recurrent SK demonstrated that the stromal inflammation in CD + 8 T cell depleted and control mice was characterized by extensive lymphocyte and polymorphonuclear leukocyte infiltration, but only occasional lymphocytes were observed in CD + 4 T cell depleted mice. Conclusion The results provide evidence that recurrent herpetic SK represents an immunopathologic process mediated by CD + 4 T cells.更多还原