【摘要】 目的　了解VITEK AMS检测大肠埃希菌和肺炎克雷伯菌产超广谱 β 内酰胺酶 (ESBLs)的可靠性 ,并调查本院这两种菌的产ESBLs情况。方法　用VITEK AMS专用药敏卡GNS 5 0 6进行ESBLs检测 ,并以头孢曲松、头孢噻肟、头孢他啶和氨曲南为底物 ,用纸片协同试验作对照。结果　仪器检测 48株肺炎克雷伯菌中有 2 4株ESBLs为阳性 ,用纸片协同法对照结果一致。检测 10 2株大肠埃希菌中 ,仪器检出 41株阳性 ,纸片协同法对照也为阳性 ,但仪器检测的 6 1株阴性菌中 ,纸片协同法对照有 19株为阳性。结论　VITEK AMS检测本地区产ESBLs菌株尚可能存在一定的局限性 ,建议各地根据感染菌株的特点及三代头孢菌素的使用情况 ,用多种底物检测ESBLs更多还原

【Abstract】 Objectives To investigate the reliability of VITEK AMS for the detection of strains of E.coil and K.pneumoniae that produce ESBLs, and to determine the prevalence in our hospital of the two strains producing ESBLs.Method VITEK GNS 506 was used to detect ESBLs, and was compared with double disk synergy test. Results Among 48 strains of K.pneumoniase detected using VITEK GNS 506, 24 strains were found to be ESBLs positive, and 24 strains ESBLs negative. The result was consistent with that of double disk synergy test. Among 102 strains of E.coli detected using VITEK GNS 506, 41 strains were found to be ESBLs positive. The result was consistent with that of double disk synergy tests. 61 strains were found to be ESBLs negative detected using VITEK GNS 506. Among them, 19 strains were found to be ESBLs positive.Conclusion Detection ESBLs using VITEK AMS has some limitations in our area. Simple and reliable method should be used more with indicates for detection of ESBLs suitable for routine screening.更多还原