【摘要】 目的　探讨结核分枝杆菌 (结核菌 )对链霉素 (Sm)产生耐药性的分子机制 ,建立直接快速检测结核菌Sm药物敏感性的试验方法。方法　应用聚合酶链反应 单链构象多态性 (PCR SSCP)银染分析技术检测 32株结核菌临床分离株的rpsL基因。结果　以结核菌标准株 (H37Rv)为对照 ,32株结核菌临床分离株中 ,10株Sm敏感株的rpsL基因未见SSCP泳动异常 ,2 2株Sm耐药株中 ,16株(72 .7% )的rpsL基因出现SSCP泳动异常。结论　rpsL基因突变是结核菌对链霉素产生耐药性的重要分子机制 ,PCR SSCP银染技术可能成为快速检测结核菌Sm药物敏感性的新方法。更多还原

【Abstract】 Objectives To study the molecular mechanism of M. tuberculosis resistance to streptomycin, and to set up a method for direct, rapid detection of streptomycin resistance mutations in M. tuberculosis. Method The rpsL genes of 32 M. tuberculosis clinical isolates were tested by PCR SSCP. Results Strain H 37 R v was used as a control. In 32 M. tuberculosis clinical isolates, the rpsL PCR fragments form 10 drug susceptible isolates had no differences in rpsL PCR profiles with strain H 37 R v. In 22 streptomycin resistant isolates,16 showed apparent differences in SSCP profiles.Conclusions The rpsL gene mutation is an important mechanism of M. tuberculosis resistance to streptomycin. PCR SSCP might be used as a simple, rapid and reliable diagnostic method for M. tuberculosis drug resistance.更多还原