【摘要】 目的　探讨ob基因对早期发育的影响及其在发育早期的显性程度。方法　用聚合酶链反应和限制性内切酶法检测C5 7BL/ 6Job小鼠的 3种基因型 :野生型 (+ / + )、杂合体型 (ob/ + )及突变型 (ob/ob)。记录小鼠出生 4d～ 2个月的体重。比较出生后 7、14和 2 4d小鼠的腹股沟白色脂肪(WAT)和背肩岬的褐色脂肪 (BAT)湿重。结果　小鼠出生 2周后 ,ob/ob小鼠体重快速增长 ,7周时已是 + / +和ob/ +小鼠体重的 2倍 ;2 4d时的WAT明显高于 + / +和ob/ +小鼠。ob基因型对BAT的影响早于WAT。ob/ +小鼠的体重和WAT均介于 + / +小鼠和ob/ob小鼠之间。 2周以前 ,ob基因对体重影响的显性指数在 1 0～ 0 5之间 ,表现为共显性 ,6周时 ,显性指数接近 0 0 ,几乎为完全隐性。结论　ob基因型对体重和WAT的影响始于第 2周后 ;ob基因对体重影响的显性指数随着年龄增加由显性向隐性发展更多还原

【Abstract】 Objective To study the effects and the extent of dominance of ob gene on early development in mice. Methods ob genotypes, wild type (+/+), hybrid type ( ob /+) and mutant type ( ob / ob ), were detected by polymerase chain reaction and restrict endonuclease technique. Body weight of mice was recorded daily for the first seventh weeks of life. Their wet weight of white adipose tissue (WAT) and brown adipose tissue (BAT) was compared at the 7th, 14th and 24th days after birth. Results Body weight of ob / ob mice increased rapidly two weeks after birth and reached two times of that of +/+ and ob /+ mice seven weeks after birth. WAT of ob / ob mice was significantly higher than that of +/+ and ob /+ mice 24 days after birth. Effect of ob genotype on BAT was earlier than that on WAT. Body weight and WAT of ob /+ mice ranged between those of +/+ and ob / ob mice. Dominance index, reflecting the effect of ob gene on body weight, ranged from 1 0～0 5 within the first two weeks after birth, appearing co dominance. And, dominance index approximated to 0 0 six weeks after birth, appearing almost complete recession. Conclusion Effect of ob gene on body weight and WAT initiated two weeks after birth and increased with age, developing gradually from co dominance to recession in their early life.更多还原