【摘要】 目的　构建一种能在卵巢癌肿瘤组织中进行肿瘤特异性表达的逆转录病毒载体 ,增强肿瘤基因治疗的靶向性。方法　PCR法获得 HER2基因的启动子片段并将其克隆至逆转录病毒载体 PL XSN中 ,再将突变型绿色荧光蛋白 (mt GFP)基因克隆至该启动子下游 ,将此载体转染 SK- OV3细胞和 MCF- 7细胞。结果　转染 3天后可见部分 SK- OV3细胞呈现绿色荧光 ,而 MCF- 7中无此现象。结论　 HER- 2基因启动子可控制报告基因在卵巢癌细胞系中进行高效特异性表达 ,此载体经修饰后可用作卵巢癌肿瘤特异性基因治疗的研究工具更多还原

【Abstract】 Objective Construct a retrovirus vector that expresses the downstream gene specifically in ovarian cancer cells so as to increase the specificity of gene therapy. Methods HER2 promoter obtained by PCR was cloned into P LXSN ; mutant GFP cDNA was inserted just downstream of this promoter. Transfect SK OV3 and MCF 7 cell lines with the new vector, P LHGSN . Results Three days after transfection with P LHGSN , green fluorescence was observed in SK OV3 cells while not in MCF 7 cells. Conclusion HER2 promoter can control tumor specific expression of the reporter gene in ovarian cancer cell lines. With proper modification, the vector is expected to be useful in tumor specific gene therapy of ovarian cancers.更多还原