【摘要】 目的　应用一种能快速、准确识别胃癌标记染色体来源的技术 ,以提高对胃癌细胞复杂染色体畸变辨认的能力。方法　采用改良的 G显带染色体标本脱色后进行荧光原位杂交 (fluorescence in situhybridization,FISH) ,分别对胃癌细胞系 SGC- 790 1的两条标记染色体 (M1和 M2 )和一例原发性胃癌的标记染色体 (M3)进行分析。结果　显示了 M1、M2和 M3有复杂的染色体结构畸变 :del(7) (p15 ) / del(7)(q2 2 ) ;t(1;3) (p11;q11)和 del(7) (q32 )。结论　该方法具有信号强、背景低和重复性好等优点 ,在识别胃癌染色体复杂结构改变中具有重要的作用。更多还原

【Abstract】 Objective Using a rapid, accurate method that detects the marker chromosomes of gastric cancer and enhancing the ability of discriminating complicated chromosome rearrangements of gastric cancer. Methods The improved method of fluorescence in situ hybridization(FISH) performed on the decolorized G banding chromosome was used. Results The changes of two marker chromosomes (M1, M2) of the cell line(SGC 7901) of gastric cancer and one marker chromosomes(M3) of one primary gastric cancer were respectively analyzed by this method. The M1, M2 and M3 had complicated structural chromosome aberrations: del(7)(p15)/del(7)(q22), t(1;3)(p11;q11) and del(7)(q32). Conclusion This method showed strong signals, low backgrounds and well repetitions. It may play an important part in exploring the chromosome rearrangements in the process of pathogenesis and development of gastric cancer.更多还原