【摘要】 目的　研究汉族人群中 139个微卫星位点的杂合度和多态信息量 (polymorphisminformation content,PIC)。方法　应用多重 PCR方法对 139个位点进行扩增。聚丙烯酰胺凝胶电泳分离 ,Gene Scan TM、Genotyper TM软件进行基因分型。结果　这些微卫星位点的杂合度为 0 .35～ 0 .89,其中 88%的杂合度 >0 .6 0 ,PIC为 0 .32～ 0 .88,94%位点的 PIC>0 .5 0。结论　微卫星位点有较高的多态信息量 ,在不同群体间存在一定的差异 ,在遗传分析中有广泛的应用价值。更多还原

【Abstract】 Objective To determine the heterozygosity and polymorphism information content of 139 microsatellite loci in Han population. Methods Multiplex approach was used to analyze the 139 loci. The amplified fragments were subjected to electrophoresis in PAGE gel and analyzed with Genescan TM and Genotyper TM . Results The heterozygosities are between 0.35 to 0.89, with the heterozygosites of 88% loci >0.60. PICs are in the range of 0.32 and 0.88 ,with PICs of 95% loci> 0.50. Conclusion The polymorphism information content of microsatellite loci is very high and their distribution varies in different races and populations.更多还原