【摘要】 目的　探索特定基因操纵区CpG岛高甲基化对人类造血系统恶性肿瘤的作用。方法　利用甲基化特异性聚合酶链反应 (MSP)的方法 ,用亚硫酸氢钠修饰后的DNA为模板进行甲基化特异性聚合酶链反应 (PCR) ,测定了 2 5例急性髓系白血病 (AML) ,15例慢性粒细胞白血病 (CML) ,16例骨髓增生异常综合征 (MDS)和 12例多发性骨髓瘤 (MM)患者P1 5INK4B基因在操纵区 5′ CpG岛异常甲基化的发生率。结果　P1 5INK4B基因异常甲基化的发生率 :AML为 84% ,CML为 0 ,MDS为 5 0 % ,MM为 75 %。高危型的MDS较低危型更易发生甲基化。在MM中 ,P1 5INK4B甲基化一般发生在早期 ,与骨髓象的幼稚程度关系密切。结论　调节细胞生长和分化的P1 5INK4B基因高甲基化是使P1 5INK4B 基因失活的主要原因之一 ,CpG岛高甲基化与恶性血液病的发生密切相关。更多还原

【Abstract】 Objective To study the effect of operative region hypermethylation gene in human malignant hematopoietic tumors. Methods The abnormal methylation rate of P 15 INK4B gene 5′ CpG island in 68 cases of malignant hematopoietic tumor samples were determined by methylation specific PCR using bisulfite modified DNA. Results The methylation rates of P 15 INK4B were 84%,0,50%and 75%, respectively, for 25 cases of acute myeloid leukemia(AML), 15 chronic myeloid leukemia(CML), 16 myelodysplastic syndrome (MDS) and 12 multiple myeloma(MM). P 15 INK4B gene was frequently methylated in patients with high risk MDS and early stage of MM. Conclusion Hypermethylation of P 15 INK4B gene is one of the main causes of its inactivation. Hypermethylation of CpG island was closely related to the development of malignant hematopoietic diseases.更多还原