【摘要】 目的　观察急性缺氧时内皮细胞中血管性血友病因子 (vWF)的变化及其与血液高凝状态之间的关系 ,确定其在反映血管内皮细胞损伤和血液高凝状态研究中的价值。方法　用免疫组织化学染色和显微分光光度计及ELISA方法分别对急性缺氧所致大鼠肺血管内皮细胞和血中vWF的含量进行检测 ,并用磷钨酸苏木素染色法显示肺血管中纤维蛋白的含量。结果　随着缺氧时间的延长 ,内皮细胞的vWF含量呈递减趋势 ,血中vWF的含量呈递增趋势 ,血管中的纤维蛋白逐渐增多。结论　急性缺氧可引起血管内皮细胞损伤 ,vWF释放入血增加 ;血中vWF的含量与血液高凝状态之间存在相关性 ,因而vWF可以作为反映血管内皮细胞损伤和血液高凝状态的指标更多还原

【Abstract】 Objective To investigate changes of vWF in vascular endothelial cells with acute hypoxia and assess its significance in the study of vascular injury and hypercoagulability. Methods vWF in vascular endothelial cells and in blood were measured by immunohistochemical staining image analysis and ELISA technique. Fibrin was displayed by phosphotungstic hematoxylin staining. Results The amount of vWF in vascular endothelial cells decreased progressively, while in blood increased progressively with hypoxica. Fibrin prominently increased with hypoxia. Conclusion Acute hypoxia caused severe injury of vascular endothelial cells which resulted in release of vWF into blood from endothelial cells. The vWF amounts in blood may reflect the hypercoagulability, and may serve as a useful indicator of vascular injury and hypercoagulability.更多还原