【摘要】 目的　观察云南静脉药瘾 (IDUs)HIV 1分离株env基因V3环顶端四肽氨基酸和相应核苷酸序列随时间推移的变化。方法　根据 1990～ 1997年间 6 2株HIV 1分离株env基因C2 V3区DNA序列 ,对HIV 1膜蛋白V3环顶端四肽基因序列 (基序 )及其编码核苷酸进行分析 ,并探讨其随时间推移的变化趋势。结果　 1990～ 1997年间 6 2株HIV 1毒株膜蛋白V3环顶端四肽基序有程度不同的氨基酸变异 ,主要表现在第四位氨基酸的改变 ,变异呈现一定的趋向性。 6 2株云南标本中有 46例(74 2 % )为GPGR ,10例 (16 1% )为GPGQ ,4例 (6 4% )为GPGK ,另有 2例 (3 3% )为GPER且四肽基序编码核苷酸的变异主要表现为A→G的改变。结论　 1990～ 1997年这一时期内 ,云南IDUs特定感染者范围内HIV 1分离株膜蛋白V3环顶端四肽序列已表现出较大的趋向性 ,符合HIV 1国际B亚型SF2株的GPGR模式更多还原

【Abstract】 Objective To investigate the variation trend of the amino acid and the corresponding nucleotide sequences of the tetrapeptide on the tip of the V3 loop of HIV 1 strains from intravenous drug users (IDUs) in Yunnan as time goes by. Methods According to the nucleotide sequences of the C2 V3 region of 62 HIV 1 strains isolated from intravenous drug users (IDUs) of Yunnan from 1990 to 1997, the nucleotide sequences of the tetrapeptide on the tip of the V3 loop of HIV 1 envelope protein were analysed. The variation trends with time were discussed. Results The tetrapeptides on the tip of the V3 loop of 62 Yunnan HIV 1 strains showed different amino acid variation with the major one at the fourth amino acid. Moreover, the variation tend to be in a similar pattern. Among the 62 isolated strains, 46 (74.2%) showed the GPGR tetrapeptide motif, 10 (16.1 %) GPER motif, 4 (6.4%) GPGK motif and 2 (3.3%) GPER motif respectively. The variation at corresponding nucleotide sequence of the tetrapeptide motif was A→G. Conclusion In the period of 1990 1997,the tetrapeptides on the tip of the V3 loop of the HIV 1 strains from infected IDUs people in Yunnan are similar, and the results conform to the GPGR motif pattern of the international B subtype HIV 1SF 2 strain.更多还原