【摘要】 目的　研究重组BCG(recombinantBCG ,rBCG)疫苗和人结核杆菌热休克蛋白 (heatshockprotein ,HSP) 6 5DNA疫苗对小鼠免疫应答的影响 ,评价两种疫苗的免疫原性。方法　用rBCG疫苗和HSP6 5DNA疫苗免疫BALB/c小鼠 ,免疫 8周时 ,采血、取腹腔巨噬细胞和脾脏进行实验。以NO释放量检测巨噬细胞吞噬活性 ,以淋巴细胞刺激指数 (SI)反映细胞增殖能力 ,以ELISA试剂盒检测血清及脾淋巴细胞培养上清的IL 2和IFN γ的含量。结果　rBCG疫苗以 10 6CFU/鼠的剂量经皮下免疫小鼠后 ,其脾淋巴细胞的增殖能力明显高于对照组 ,BCG组和HSP6 5DNA疫苗组 (P <0 .0 5 ) ;腹腔巨噬细胞一氧化氮 (NO)的含量和血清IL 2的含量明显高于对照组 (P <0 .0 1) ;血清IFN γ的含量明显高于HSP6 5DNA疫苗组 (P <0 .0 5 ) ;其余测定参数均有升高趋势 ,但差异无显著性 (P >0 .0 5 )。而HSP6 5DNA疫苗以 5 0 μg/鼠的剂量经肌注免疫小鼠后 ,其血清IL 2的含量明显高于对照组 (P <0 .0 1) ;血清IFN γ的含量明显低于rBCG组 (P <0 .0 5 ) ;其余测定参数与其他各组相比差异均无显著性(P >0 .0 5 )。结论　本实验结果显示 ,rBCG疫苗具有良好的免疫原性 ,能明显增强BALB/c小鼠的免疫应答反应。HSP6 5DNA疫苗亦能刺激机体的免疫应答 ,但其反应强度似乎不更多还原

【Abstract】 Objective The aim of this study is to evaluate the immunogenicity of recombinant BCG(rBCG) and human Mycobacterium tuberculosis heat shock protein(HSP)65 DNA vaccine. Methods The specific pathogen-free BALB/c mice were immunized with rBCG or HSP65 DNA vaccine by sc or im. The lymphocyte stimulating index(SI), macrophage activity as well as IL-2, IFN-γ levels of the serum and culture supernatant of spleen lymphocyte were tested by the end of 8th week of inoculation. Results After immunization with rBCG vaccine in dose of 10 6 CFU/per capita by sc, the SI was significantly higher than those in the control group, BCG and HSP65 DNA groups ( P <0.05); the macrophage activity and serum IL-2 level were also significantly higher than those in the control group ( P <0.01);the serum IFN-γ level was significantly different from that of HSP65 DNA group ( P <0.05); Compared with each groups. Other parameters were no significantly different( P >0.05). After immunization with HSP65 DNA vaccine in dose of 50μg/per capita by intramuscular injection, the serum IL-2 level was significantly higher than that in the control group ( P <0.01);the SI and serum IFN-γ level were significantly lower than that in the rBCG group( P <0.05);compared with each groups, the macrophage activity and the culture supernatant IL-2 level of spleen lymphocyte were no significantly different( P >0.05). Conclusions The rBCG vaccine may induced stronger immune response in BALB/c mice than did the BCG and HSP65 DNA vaccine, whereas the HSP65 DNA vaccine may induced the immune response of BALB/c mice, but its stimulating effect is lower than that by the rBCG vaccine.更多还原