【摘要】 目的　探讨在前列腺癌中CD44和nm2 3 H1基因表达的意义。　方法　应用免疫组织化学、银染单链长度构相多态性半定量逆转录聚合酶链式反应及免疫印迹杂交法分别检测 32例前列腺癌石蜡标本及 15例新鲜标本中CD44、nm2 3 H1基因的突变、表达情况。　结果　前列腺癌组织中nm2 3 H1蛋白及mRNA高水平表达 ,转移组nm2 3 H1蛋白表达强度高于非转移组。前列腺癌组织中存在nm2 3 H1基因突变 ,突变检出率为 13 3% (2 / 15 )。前列腺癌转移组中 ,CD44s蛋白表达水平下降。CD44smRNA在癌及非癌对照组织中全部表达 ,但 86 7% (13/ 15 )的癌组织中同时表达CD44v。　结论　nm2 3 H1基因的高表达和CD44v/CD44s基因的表达失衡可能在前列腺癌的恶性进展中共同发挥作用。更多还原

【Abstract】 Objective[WT5”BZ] To study the role of nm23 H1 and CD44 gene in carcinogenesis and metastasis of prostate cancers. [WT5”HZ]Methods[WT5”BZ] Expression of nm23 H1/NDPK and CD44 protein were analyzed in 32 cases of prostate cancers (Pca)and 12 control of benign prostate hyperplasia(BPH) paraffin embedded tissue by immunohistochemistry. Mutation and expression of their genes were detected by silver SSCP method, semiquantitative RT PCR, and southern blot hybridization analysis respectively in 25 cases of fresh Pca and 15 control BPH tissue. [WT5”HZ]Results[WT5”BZ] nm23 H1 gene point mutation was detected in 13 3%(2/15) of Pca. Compared with the control tissue, the expression of nm23 H1 gene was increased significantly on both mRNA and protein levels in the carcinoma tissue( P <0 05). The expression of nm23 H1 protein was also increased significantly in Pca with metastasis. The expression of CD44 standard form (CD44s) protein was significantly reduced in most Pca compared with BPH tissue. CD44s mRNA was detected in all Pca and BPH tissue, whereas CD44 variant (CD44v) without CD44v6 was mainly detected in carcinoma tissue. [WT5”HZ]Conclusions[WT5”BZ] The altered expression of CD44s and CD44v may coregulate in the carcinogenesis and progression, whereas nm23 H1 gene may play a different role from CD44 in the progression of prostate cancer. CD44v6 may be a useful but not significant tool for detection of colon or bladder cancers. [WT5”HZ]更多还原