【摘要】 目的　明确Fas配体在泌尿生殖肿瘤细胞系及肾癌组织中的表达情况。　方法　采用免疫细胞化学和反转录PCR(RT PCR)法检测膀胱癌 (T2 4 、BIU 87、EJ)、肾癌 (GRC 1、RCC 949)、前列腺癌 (PC 3M)及 10例肾癌组织中Fas配体的表达。　结果　免疫细胞化学方法检测细胞系中Fas配体表达于BIU 87、RCC 949和GRC 1细胞 ,而以BIU 87和RCC 949细胞系表达较强 ,在T2 4 、EJ和PC 3M细胞系中未检测到Fas配体的表达 ;在 6种泌尿生殖肿瘤细胞系中用RT PCR方法证明Fas配体mR NA表达于 4种肿瘤细胞系 (GRC 1、RCC 949、BIU 87、PC 3M) ,而EJ与T2 4 未检测到Fas配体mRNA的表达 ;RT PCR检测 10例肾癌组织中有 8例存在FasLmRNA的表达。　结论　Fas配体在泌尿生殖肿瘤细胞系及肾癌组织中表达可能参与了恶性肿瘤的形成和进展更多还原

【Abstract】 Objective To explore the expression of Fas ligand in urogenital malignant cell lines and renal cell carcinoma. Methods With immunocytochemistry and reverse transcription polymerase chain reaction (RT PCR), we detected the expression of Fas ligand (FasL) in 6 urogenital malignant cell lines, bladder carcinoma cell lines (T 24 , EJ, BIU 87), renal cell carcinoma cell lines (GRC 1, RCC 949),prostatic carcinoma cell line (PC 3M), and 10 cases of renal cell carcinoma. Results The expression of FasL was detected in 3 (BIU 87, RCC 949, GRC 1) of the 6 urogenital maligant cell lines with immunocytochemistry, and 4 (BIU 87, RCC 949, GRC 1, PC 3M) of 6 urogenital maligant cell lines with RT PCR method. No expression of FasL was detected in bladder carcinoma cell lines T 24 and EJ. Eight of 10 renal cell carcinoma tissues positively expressed FasL mRNA. Conclusions FasL can express in urogenital malignant cell lines and renal cell carcinoma.更多还原