【摘要】 目的　研制可诱导的、耗时较短的慢性移植物抗宿主病（ ＧＶＨＤ）狼疮样肾炎小鼠模型。方法无菌分离母体 ＤＢＡ／２ 小鼠淋巴细胞，注入雌性（Ｃ５７ＢＬ／１０ × ＤＢＡ／２） Ｆ１杂交鼠。观察１６周。结果注射后８周模型小鼠以肾小球细胞增生为主要特征，未见蛋白尿及间质损害；注射后１２周以大量蛋白尿、低蛋白血症、水肿及高脂血症为其主要症状，病理以肾小球系膜增宽、基底膜节段性增厚、肾间质细胞浸润为特点；至注射后１６周则以肾功能损害为主要改变，病理以肾小球基质大量增生，肾间质纤维化为主要特征。血清学示抗ｄｓＤＮＡ抗体阳性，免疫荧光示免疫球蛋白及补体沿系膜（ＩｇＭ）和毛细血管（ＩｇＧ、Ｃ３）沉积及电镜可见上皮下电子致密物沉积提示本模型符合ＬＮ的特点。结论慢性ＧＶＨＤ狼疮样小鼠模型病理改变类似人类狼疮肾炎（ＬＮ），且省时、变异小，病变呈明显的阶段性。该模型为研究ＬＮ的发病机制、预防和治疗提供了良好的工具。更多还原

【Abstract】 Objective To establish chronic graft-versus-host disease (GVHD) inducing murine lupus nephritis(LN) model. Methods (C57BL/10 × DBA/2) F1 hybrids were used as recipients of DBA/2 (donor lymphocytes. Single cell suspensions were injected intravenously 4 times at 3 - 4 days intervals. Results Eight weeks after injection of lymphocytes, the glomerular cells increased significantly, and no cast and protein appeared in urine. At twelfth week, glomerular mesangial segmental proliferation, extracellular matrix expansion and interstitial damage exhibited. Albuminuria aggravated markedly in affected animals, causing hypoalbuminemia, hyperlipidemia and edema. At sixteenth week, global glomerular sclerosis appeared and uremia developed. Deposits of immunoglobulins were observed along the glomerular capillary wall and in the mesangium. Electron microscopy revealed the presence of subepithelial electron-dense deposits. Anti-dsDNA autoantibody titers increased significantly during 8 to 16 weeks after GVHD induction. Conclusion The murine model is comparable to the lesions characterizing human LN and of offers an excellent model for studies on the pathogenesis, prevention and treatment of LN.更多还原