【摘要】 目的　探讨人巨细胞病毒 (HumanCytomegalovirus,HCMV)感染大鼠的敏感性和稳定性 ,探讨建立大鼠感染HCMV动物模型的可行性。方法　第一次动物试验 :SD大鼠 30只 ,随机均分为 3组 ,分别为接种病毒组、灭活病毒组和正常对照组 ,病毒接种途径为尾静脉注射 ;第二次动物试验 :Wistar大鼠 40只 ,分别为接种病毒组 (2 0只 )、灭活病毒组 (2 0只 ) ,病毒接种途径同第一次动物试验。两次试验动物均于 90d后分别以病理学方法研究动物组织损伤特点 ,以免疫组化方法检查病毒抗原 ,原位杂交方法检测动物组织细胞中HCMV基因。结果　两次静脉途径接种HCMVAD169株 90d的动物 ,组织发生广泛病理损害 ,免疫组化方法和原位杂交方法在多种组织细胞内查到HCMV抗原和基因表达。结论　HCMVAD169株可感染健康大鼠 ,可望作为HCMV动物慢性感染模型更多还原

【Abstract】 Objective To Study the sensitivity of rat to human cytomegalovirus (HCMV) infection and to establish a stable animal model.Methods Two kinds of rat experiments were carried out:1)30 SD rats were divided into three groups randomly,they were virus inoculated group,inactivated virus inoculated group and the normal rat group respectively; 2)40 Wistar rats were randomly divided into two groups,they were the virus inoculated group and the inactivated virus inoculated group.All the rats were inoculated through tail vein.90 d later,the rat’s tissue lesions were observed by pathological techniques,HCMV antigen was detected by immunohistochemical method and the HCMV DNA was analyzed by in situ hybridization. Results The extensive pathological damages in tissues of HCMV infected rats were observed.Meanwhile,the viral antigen and viral DNA were also demonstrated in many tissues of HCMV infected rats.Conclusion Rat is sensitive to infection of HCMV AD 169 strain, but, as an animal model study,we still have a lot of works to do.更多还原