【摘要】 目的　调查TTV阴性的非甲～庚型肝炎患者中TTV -likeminivirus(TLMV)感染情况 ,对TLMV5′非编码区 (5′NCR)部分基因进行分子克隆与序列分析。方法　采用巢式PCR技术检测5 3例TTV阴性的非甲～庚肝炎患者血清TLMVDNA ,对PCR产物进行克隆、测序和分析。结果　 5 3例病例中TLMVDNA阳性 37例 (6 9 8% ) ,对其中 8株TLMV基因克隆测序 ,并与Takahashi报道的TLMV序列 (GenBankAccessionNo ab 0 2 6 930、ab0 2 6 931)比较 ,其核苷酸序列同源性在 6 4%～ 83%之间。结论　在TTV阴性的非甲～庚肝炎患者中存在TLMV感染。TLMV5′NCR基因变异性较大。TLMV的致病性及其与非甲～庚肝炎的关系尚有待进一步研究更多还原

【Abstract】 Objective To investigate the TTV-like mini virus(TLMV) infection in Chinese patients with non A-G hepatitis and to analyse sequence of 5′ non-coding region(5′NCR)of TLMV.Methods TLMV DNA was detected by nested-PCR from 53 serum samples of non A-G hepatitis patients without TTV infection.PCR products were cloned and sequenced. Results TLMV DNA were positive in 37 of 53 patients(69.8%).The sequence of 5′NCR of 8 TLMV isolates was compared with that reported by Takahashi(Gen Bank Accession No. ab 026930-026931)and showed nucleotide identity between 64%-83%.Conclusion The results of this study indicated that TLMV infection is common in TTV negative patients with non A-G hepatitis.The 5′NCR sequence of TLMV showed a great degree of diversity.The pathogenicity of TLMV and its relationship to non A-G hepatitis need to be confirmed and further study is required.更多还原