【摘要】 目的　通过病毒粒 7个不同RNA节段核苷酸序列测定和分析 ,进一步排除A/广东 / 6 /91(H1N1)毒株由实验室污染而来。它与A/PR/ 8/ 34 (H1N1)毒株基因组间有哪些RNA节段存在有差异。同时它是否是一株基因重配株 ?方法　病毒粒RNA经逆转录合成cDNA ,经聚合酶链反应 (PCR)扩增 ,产物纯化 ,采用双脱氧链末端终止法进行核苷酸序列测定。结果　所比较的 7个不同RNA节段中 ,测定毒株RNA6和 7的核苷酸序列与A/PR/ 8/ 34 (H1N1)完全相同。而RNA1,2 ,3,5和 8间核苷酸差异数分别为 2 0 ,5 ,11,7和 6。这些差异导致了所编码的PB2 ,PB1,PA ,NP和NS蛋白分子上氨基酸序列差异数分别为 10 ,2 ,1,0和 4。结论　A/广东 / 6 / 91(H1N1)毒株由A/PR/ 8/ 34 (H1N1)病毒实验室污染而来可完全加以排除。它是A/PR/ 8/ 34 (H1N1)病毒类似毒株 ,而不是基因重配株。更多还原

【Abstract】 Objective Through the test and analysis of nucleotide sequences of seven different RNA segments of the virion to exclude the possibility that A/Guangdong/6/91(H1N1)strain might be derived from laboratory contamination and to determine how many RNA segments are there with nucleotide sequence difference between A/Guangdong/6/91(H1N1) and A/PR/8/34(H1N1) viruses, as well as to determine is A/Guangdong/6/91 virus a reassortant?Methods Virion RNA was transcribed into cDNA by reverse transcriptase, cDNR was amplified by PCR, the products of PCR were purified. Afterward, RNA sequence test was performed by the dideoxynucleotide chain termination method using synthetic oligodeoxynucleotide primers.Results There are no nucleotide sequence differences in RNA segments 6 and 7 between A/Guangdong/6/91(H1N1) and PR8 strains, while there are 20,5,11,7 and 6 differences in nucleotide sequences of RNA segments 1,2,3,5 and 8 between these two strains, respectively. These differences induce 10,2,1,0 and 4 differences in amino acid sequences on PB2,PB1,PA,NP and NS protein molecules between A/Guangdong/6/91(H1N1) and PR8 strains, respectively.Conclusion The possibility of laboratory contamination of A/Guangdong/6/91(H1N1) virus can be ruled out completely.A/Guangdong/6/91(H1N1) virus is a PR8 like strain, but not a reassortant.更多还原