【摘要】 目的　对人源汉坦病毒疫苗候选株作分离鉴定及特性的研究。方法　用Vero细胞分离病毒 ,用免疫荧光法作病毒效价测定。用RT PCR及部分核苷酸序列分析等检定。结果　用Vero细胞从安徽省凤台县肾综合征出血热病人血清中分离一株汉坦病毒 (HV) ,其抗原特性及PCR分型结果证明为汉滩病毒 (Ⅰ型病毒 ) ;其免疫血清对来源于不同地区的HV均有中和活性。M片段部分序列分析结果表明 ,该毒株与 76 118等毒株核苷酸序列有较大差异 ,但氨基酸序列同源性甚高。病毒株对Vero细胞敏感 ,繁殖高峰一般在 9天以后 ,可多次收获病毒。结论　上述特性表明 ,分离病毒株适合用作研制灭活疫苗后备株。更多还原

【Abstract】 Objective Isolation and identification of a Hantaan virus from HFRS patient’s serum for killed vaccine development.Methods This virus temporally called FT14 detected by IFAT, RT PCR. Partial sequence of M segment was determined by Sanger’s dideoxy chain termination method.Results The antigenicity and PCR amplification of the newly isolated virus FT14 proved that it belongs to virus of HTN type. Antiserum to the virus can neutralized many strains of Hantaan virus from different areas. The partial sequence of M segment of FT14, as compared with other HTN strains, showed great differences among nucleotied sequences, while the deduced amino acid sequence is comparatively conservative. The titer of the virus reached its peak at 9 days post inoculation in vitro , then the peak titer could last for a long time. Conclusion This virus strain FT14 would be a good candidate virus for killed vaccine development.更多还原