【摘要】 目的研究积雪草甙对体外培养的成纤维细胞的胶原蛋白合成的影响，以及对裸鼠增生性瘢痕动物模型的作用，以探讨积雪草甙治疗增生性瘢痕的机制。方法采用光镜、电镜研究用药前后成纤维细胞的形态学的变化，以~３Ｈ脯氨酸掺入等方法检测成纤维细胞胶原蛋白的合成，通过建立裸鼠增生性瘢痕动物模型，观察积雪草甙在体局部注射对增生性瘢痕的作用。结果积雪草甙能明显影响成纤维细胞的超微结构，抑制胶原蛋白的合成，并呈量效关系；在裸鼠体内局部注射积雪草甙可抑制瘢痕增生，而且大剂量使用未发现药物毒性作用。结论积雪草甙治疗瘢痕增生的机理与其抑制成纤维细胞增殖和胶原蛋白合成有关。更多还原

【Abstract】 Objective To study the effect of Asiaticoside on the fibroblast collagen biosynthesis in vitro culture and on the hypertrophic scar in a nude mice model, and its mechanism. Methods light microscope (LM ) and electron micro- scope (EM) were employed to study the morphological changes in fibroblast before and after the application of Asiaticoside. The fibroblast collagen synthesis was assayed by ~3H-proline incorporation. A nude mice model with hypertrophic scar was estab- lished for observing the effect of Asiaticoside given by local injection. Results It was found that Asiaticoside could significant- ly affect the ultrastructure of fibroblast and inhibit its collagen synthesis in vitro culture (P < 0.01) in a manner of dose-effect relationship. Local injection of Asiaticoside into the nude mouse body could inhibit the proliferation of scar without any toxic effect. Conclusion The possible mechanism of the effect of Asiaticoside on hypertrophic scars is related tO its inhibito- ry action on the fibroblast proliferation and collagen synthesis.更多还原