【摘要】 目的　了解胰腺移植术后应用雷公藤多甙对移植胰腺功能存活的影响。方法　供体为雄性Wister大鼠 ,受体为雄性SD大鼠。受体经尾静脉注射链脲霉素 5 0mg/kg制备成糖尿病大鼠模型。胰腺移植采用全胰腺、十二指肠移植 ,胰岛素回流采用门腔静脉吻合的体循环回流 ,外分泌胰液采用供受体十二指肠间侧侧吻合的肠引流法。血管吻合采用显微外科技术。胰腺移植术后开始每隔2d测 1次血糖及尿糖 ,术后第 4d测血清胰岛素 ;非空腹血糖连续 2d低于 11.2mmol/L ,尿糖反应阴性的作为移植物功能存活的标准 ;非空腹血糖连续 2d超过 11.2mmol/L ,尿糖反应阳性作为移植物功能丧失的指标。结果　移植胰腺功能存活天数如下 :对照组 (10 .4± 2 .9)d ;雷公藤多甙处理组(2 2 .0± 1.5 )d。结论　胰腺移植术后给予雷公藤多甙灌服治疗 ,能够明显延长移植胰腺的功能存活时间更多还原

【Abstract】 Objective To study the effects of tripterium wilfordii hook f. (T11) used after transplantation on survival of pancreas graft. Methods Male Wistar rats weighing 200 300?g served as donors, while recipients were male SD rats rendered diabetes with strepotozotocin administered intravenously at a dose of 50?mg/kg body weight through tail vein. Vascularized heterotopic whole organ pancreaticoduodenal transplantation was performed. The graft exocrine secretion was drained into duodenum by side to side anastomoses between the duodenum of donor and recipient. The insulin was drained into systemic vena by anastomoses between inferior vena cava and the infrarenal abnominal aorta of recipient and the donor aorta and portal vein. The vascular anastomoses were done by using microsurgical method. The non fasting serum glucose and urine glucose reaction were detected at an interval of 2 days and the serum insulin was detected at the 4th day post transplantation. Results All of the recipients had normoglycemia and negative urine glucose reaction 24?h after transplantation. The survival time of the pancreatic grafts was as follows: ( 10.4 ± 2.9 )days in group 1, ( 22.0 ± 1.5 )days in group 2. Conclusion Administration of T11 after pancreatic transplantation could prolong the pancreatic graft survival.更多还原