【摘要】 目的　观察ＣＤ２８ 共刺激信号对淋巴细胞免疫活化基因表达的影响及环孢素Ａ（ＣｓＡ）对它们的抑制作用。方法　分离健康人外周血单个核细胞， 分别给予抗ＣＤ３ 单克隆抗体（ 抗ＣＤ３ｍＡｂ） 单刺激或抗ＣＤ３ ｍＡｂ＋ 抗ＣＤ２８ 单克隆抗体（抗ＣＤ２８ ｍＡｂ）共刺激培养； 加或不加入ＣｓＡ。于培养后６ ｈ 和２４ ｈ 收集细胞。以逆转录聚合酶链反应（ＲＴＰＣＲ） 检测βＡｃｔｉｎ、ＣＤ４０Ｌ、ｂｃｌｘＬ、ＴＧＦβ１ 、Ｆａｓ、ＦａｓＬ、穿孔素和颗粒酶ＢｍＲＮＡ 表达水平。结果　ＣＤ２８ 共刺激信号能显著增强免疫活化基因ｍＲＮＡ 的表达；ＣｓＡ 对ＦａｓＬ和颗粒酶Ｂ的ｍ ＲＮＡ表达能产生抑制， 而对共刺激后其余免疫活化基因的表达无明显抑制。结论　ＣＤ２８ 共刺激信号通过提高多种Ｔ 淋巴细胞相关的免疫活化基因的表达水平， 而使免疫反应的活化、增殖和效应过程均得到增强；ＣｓＡ 对这一过程并非完全无效， 仍可能具有调节作用。更多还原

【Abstract】 Objectives To observe the effect of CD28 costimulation on lymphocyte immune activation gene expression and the suppressive effect of cyclosporin A(CsA). Methods Healthy peripheral blood mononuclear cells were isolated. During the culture, anti CD3 mAb single stimulation or anti CD3+anti CD28 mAb costimulation was given in the presence or absence of CsA. The cells were harvested 6?h and 24?h after the culture. mRNA expression of β Actin, CD40L, bcl xL, TGF β 1, Fas, FasL, Perforin and Granzyme B was detected by using RT PCR technique in the samples of CD28 costimulated lymphocytes. Results CD28 costimulation could obviously enhance the expression of immune activation genes. CsA inhibited the expression of FasL and Granzyme B, but had no obvious inhibition on the expression of immune activation gene following costimulation. Conclusions CD28 costimulation could increase the expression of various lymphocyte related immune activation gene to enhance the activation, proliferation and response of immune reaction. CsA might have modulatory effects to some extene.更多还原