【摘要】 目的　研究nm2 3 H1基因表达与胰腺癌淋巴结转移及细胞增殖状态的关系。方法　用免疫组织化学S P法 ,检测 2 9例胰腺癌标本中nm2 3 H1基因产物和增殖细胞核抗原 (PCNA)的表达状况 ,并应用图像分析技术检测胰腺癌细胞核DNA含量及DNA倍体构成。结果　在nm2 3 H1高表达的 11例胰腺癌中 ,伴有淋巴结转移 6例 ,而nm2 3 H1低表达者 18例中仅 2例伴淋巴结转移 ,两者比较 ,P <0 0 5 ;nm2 3 H1表达与肿瘤部位、大小、组织学类型及患者性别等因素无相关 (P >0 0 5 ) ;nm2 3 H1高、低表达的胰腺癌中PCNA表达指数分别为 (2 6± 14) %和 (10± 5 ) % ,两者相比差异有非常显著意义 (P <0 0 1) ;细胞核DNA指数分别为 (2 2 5± 0 5 6 )和 (1 6 7± 0 2 3) ,两者相比差异有非常显著意义 (P <0 0 1) ;在DNA倍体分布形式上 ,nm2 3 H1高表达者二倍体细胞检出率低 (P <0 0 5 ) ,而非整倍体细胞检出率高 (P <0 0 1)。结论　nm2 3 H1基因产物过度表达与胰腺癌淋巴结转移及高细胞增殖活性有关。更多还原

【Abstract】 Objective[WT5”BZ] To study the relationships between nm23-H1 gene expression and lymphatic metastasis or proliferative activity in human pancreatic carcinoma.[WT5”HZ] Methods[WT5”BZ] Expression levels of nm23-H1 gene product/NDPK and proliferating cell nuclear antigen (PCNA) were examined immunohistochemically. DNA contents and ploidy patterns were measured using image analysis system. [WT5”HZ]Results[WT5”BZ] Of 29 cases tested, 11 cases showed strong immunoactivity for nm23-H1 protein/NDPK, while 18 showed low or no immunoactivity. Strong nm23-H1 staining was found to be associated with higher incidence of lymph node metastasis ( P <0 05), and also with significantly higher DNA values ( P <0 01) or higher expression levels of proliferating cell nuclear antigen ( P <0 01).[WT5”HZ] Conclusions[WT5”BZ] Our data indicated that over-expression of nm23-H1 gene product/NDPK might be relevent to lympatic metastasis and high proliferative activity in human pancreatic carcinoma. [WT5”HZ]更多还原