【摘要】 目的　检测氯胺酮对应激大鼠c fos基因表达的影响 ,从基因水平探讨药物防治应激反应的分子机制。方法　 2 1只Wistar大鼠随机分成 3组 :对照组 (C组 )、单纯电刺激组 (S组 )腹腔分别注入生理盐水 2ml、氯胺酮处理组腹腔分别注入氯胺酮 10 0mg·kg- 1 (K组 ) ,每组 7只。注药 5min后接通电刺激仪 ,C组不通电 ,其余两组均以 2mA直流电行电刺激足底 ,刺激时限 1s,间隔 30s一次 ,持续 15min。刺激后 30min断头处死提取大脑皮质、下丘脑和海马处总RNA ,经逆转录多聚酶链反应得到cDNA扩增产物 ,用c fos基因扩增产物的密度与γ actin基因扩增产物的密度比值 ,表示c fosmRNA的表达水平。结果　S组大鼠脑组织c fosmRNA的表达水平较对照组明显升高 (P <0 0 1) ,与S组相比 ,K组海马、大脑皮质c fosmRNA表达显著降低 (P <0 0 5和 0 0 1) ,但其下丘脑c fosmRNA表达水平无统计学差异 (P >0 0 5 )。结论　c fos参与了防治应激反应的分子基因机制 ;氯胺酮对应激反应c fos基因表达的影响有部位性差异。更多还原

【Abstract】 Objective To detemine the effects of ketamine on c fos gene expression in the different brain regions of rats following stress responses Methods Twenty one male Wistar rats were randomly allocated to receiving intraperitoneally 2 ml normal saline (group C or group S,n=7) or ketamine 100mg·kg 1 (group K,n=7) respectively, 5 min later, the stress response was induced with electrical foot shock (2 0mA,1s of duration,once every 30s,over 15min)only in group S and K All rats were decapitated 30min after the stress to extract the total cellular RNA of cerebral cortex, hypothalamus and hippocampus RT PCR technique was applied to determining cDNA amplification products with γ actin mRNA being as an internal control Densities of DNA bands were quantified using image analysis system Results The c fos mRNA levels were markedly elevated in group S as compared with control levels (P<0 01) Compared with those in group S, the c fos mRNA levels in the cerebral cortex and hippocampus decreased significantly ( P<0 05 or 0 01 ),but in the hypothalamus remained unchanged in group C(P>0 05).Conclusions The c fos gene involves in molecular modulation of stress responses Ketamine produce different effects on the expression of c fos gene in the different brain regions更多还原