【摘要】 目的　通过对长寿老人人类白细胞抗原 (HLA) DQA1、DQB1基因多态性的研究 ,探讨HLA与长寿的关系。　方法　应用聚合酶链反应 序列特异引物法 (PCR SSP)对巴马县 10 9名 90岁以上长寿老人、5 6名 80岁老年人及 71名健康、无血缘关系、随机抽样的当地成年人进行HLA DQA1、DQB1基因分型 ,受检者均为壮族。　结果　 (1)长寿组HLA DQA1 0 10 2的频率明显高于对照组 (分别为 43 3%和 2 7 8% ,P <0 0 0 5 ) ;(2 )长寿组HLA DQA1 0 10 1及DQB1 0 30 1分别为 3 7%和 0 5 % ,明显低于对照组 15 2 %和 2 1% (P分别 <0 0 0 1及 0 0 5 ) ;(3)长寿组与 80岁组比较 ,各相应等位基因之间的频率无显著性差异。相关分析显示 ,HLA DQA1 0 10 2与长寿呈正相关 ,而DQA1 0 10 1及DQB1 0 30 1呈负相关 (OR分别为 2 0 0 2、0 2 0 77及 0 5 0 0 7,P分别 <0 0 0 5、<0 0 5及 <0 0 5 )。　结论　在巴马县的壮族人群中 ,HLA DQA1 0 10 2为长寿的有利因子 ,而DQA1 0 10 1及DQB1 0 30 1为长寿的不利因子。更多还原

【Abstract】 Objective To investigate the possible association between HLA antigens and longevity. Methods By using PCR SSP method to genotype the HLA DQA1 and DQB1 loci for 109 longeval subjects (90～103 years, mean 96 years), 56 octogenarians (80～89 years, mean 85 5 years) and 71 healthy, unrelated, randomly selected subjects (23～70 years, mean 54 6 years) who live in the vicinity. All the individuals tested were of Zhuang nationality in Bama county and their families had lived there for generations. Results The frequency of HLA DQA1 *0102 allele was significantly higher in the longeval group, as compared with that of the control group (43 3% and 27 8%, P<0 005), while the frequency of the DQA1 *0101 and DQB1 *0301 in the longeval subjects was 3 7% and 0 5%, significantly lower than that of the control subjects (15 2% and 3 7%, P<0 001 and 0 05, respectively). No change was found between the octogenarians and the longeval group. Our data suggest that HLA DQA1 *0102 may be an active factor, while the DQA1 *0101 and DQB1 *0301 may be the risk factors for human longevity(OR=2 002, 0 2077 and 0 5007, respectively). Conclusions HLA DQA1 *0102, 0101 and DQB1 *0301 may be involved in human longevity in Bama population.更多还原