【摘要】 目的　研究放线共生放线杆菌血清b型 (Aay4)对钛及钛 75合金种植体表面的失泽腐蚀。方法　将纯钛、钛 75合金机械加工成 10mm× 10mm× 1mm的板片 ,数量分别为 30片和 6 0片 ,取钛 75合金试件 30片与纯钛试件分别进行化学钝化。将钝化纯钛组 30片随机分成空白组、培养基组和实验组 ,每组各 10片。在厌氧环境中将Aay4接种到改良GAM培养基上 ,将试件贴附于表面 ,每周转换传递 1次 ,共 10周。用 0 0 5 %戊二醛灭菌 ,清洗 ,2周后进行色度计观察。结果　肉眼观察见纯钛、钛 75合金 (钝化与非钝化 )的实验组试件表面颜色均由银灰色变成黄色。 3种试件的空白对照与培养基对照组的L 、a 、b 值无显著变化 ;而实验组分别与空白对照组、培养基对照组比较 ,其L 、a 、b 值的差异均有显著性 (P <0 0 5 )。结论　Aay4对钛及钛 75合金 (钝化和非钝化 )均发生肉眼可见的表面失泽。色度计分析表明 ,3种类型的种植体试件表面颜色由绿黄区域落到了红黄区域更多还原

【Abstract】 Objective To study the tarnish induced by Aay4 on titanium and Ti 75 alloys Methods Pure titanium and Ti 75 alloys were machined into size of 10 mm×10 mm×1 mm in tablets(30 of pure titanium; 60 of Ti 75 alloys) Thirty specimens sampled randomly from Ti 75 alloy together with 30 specimens of titanium were subjected to chemical passivation treatment 30 specimens of the pure titanium, depassivation Ti 75 alloy and passivation Ti 75 alloy were randomly divided into three groups (blank control group, media control group and inoculated media group) respectively, every group contains 10 specimens Under the anaerobic conditions, the surfaces of the modified GAM media were inoculated with Aay4 The labeled specimens surfaces were then aseptically placed on top of the media surface for 10 weeks with one generation time per week The specimens were removed after 10 weeks of incubation, immersed in 0 05% glutaradehyde solution to eliminate Aay4, rinsed with distilled water After 2 weeks, the specimens were removed and observed visually or examined by MINOLTA CR 100 color apparatus Results Visual evaluation: Aay4 caused significant tarnish on specimens exposed to inoculated media, all the specimens surface changed into yellow color Color apparatus evaluation: this result showed that comparing with the blank control, media control has no influence on all the specimens L *a *b * values ( P >0 05) Aay4 caused significant tarnish on specimens exposed to inoculated media, the result showed that there was significant difference between media control group and blank control group with inoculated media group on all the specimens L *a *b * values ( P <0 05) Conclusion Aay4 caused significant tarnish on pure titanium, depassivation Ti 75 alloys and passivation Ti 75 alloys by naked eyes and color apparatus, according CIE1976L *a *b * color system reading, the color of titanium and Ti 75 alloys changed from the yellow green ranges to red yellow ranges更多还原