【摘要】 目的　探讨蛋白激酶C(proteinkinaseC ,PKC)与涎腺腺样囊性癌侵袭转移的关系及PKC抑制剂抗肿瘤侵袭转移的可行性。方法　采用从牛精子中提纯的内源性PKC大分子抑制剂 (proteinkinaseCinhibitorofbovinespermatozona ,BS PKCI)及外源性抑制剂H7作用于人涎腺腺样囊性癌SACC 83系细胞 ,观察两种PKC抑制剂对肿瘤细胞与基底膜的粘附性、移动性 (用移出琼脂糖滴数及最远距离表示 )及体外侵袭力的影响。结果　①两种PKC抑制剂可明显降低肿瘤细胞对基底膜的粘附性 (P<0 .0 5 ) ;②两种PKC抑制剂可明显地降低肿瘤细胞的移动性 (P <0 .0 5 ) ;③两种PKC抑制剂可明显降低肿瘤细胞的体外侵袭力 (P <0 .0 1)。结论　PKC与涎腺腺样囊性癌侵袭转移有密切的关系 ;PKC抑制剂BS PKCI及H7可明显地降低SACC 83系细胞粘附性、移动性及体外侵袭力更多还原

【Abstract】 Objective To explore the relationship between protein kinase C ( PKC ) and invasion/metastasis of salivary adenoid cystic carcinoma and to explore the possibility of treatment against invasion/metastasis of the tumor cell by PKC inhibitor. Methods SACC 83 cell line cells were treated by endogenous PKC inhibitor BS PKCI extracted form bovine spermatozona and exogenous PKC inhibitor H 7, while the adhesion to basement membrane , movement ( expressed by the cell numbers moving out from agarose drop and the furtherest distance ) and invasive capacity in vitro were observed .Results (1) The two inhibitors supressed significantly adhesion of the tumor cell to basement membrane ( P < 0.05) . (2) The two inhibitors supressed significantly movement of the tumor cells ( P < 0.05 ) . (3) The two inhibitors supressed significantly invasive capacity of the tumor cells in vitro ( P < 0.01 ). Conclusions There was a close relationship between PKC and invasion/metastasis of salivary adenoid cystic carcinoma ;the two PKC inhibitor supressed significantly adhesion , movement and invasive capacity of SACC 83 cell line cells in vitro .更多还原