【摘要】 目的　研究胃癌组织内血管内表皮生长因子 (vascularendothelialgrowthfactor ,VEGF)及其受体Flt、KDRmRNA的表达及与胃癌生物学行为的相关性。方法　应用半定量逆转录聚合酶链反应(RT PCR)法检测了 6 0例手术切除、病理证实的胃癌组织内的VEGF及受体Flt、KDRmRNA。结果　VEGF、Flt、KDRmRNA在胃癌组织内的阳性表达率分别为 73.3%、86 .7%和 80 .0 % ,明显高于癌旁组织 43.3%、33.0 %和 30 .0 % (P <0 .0 1) ;伴有淋巴结转移组胃癌的VEGF、Flt、KDR的阳性表达率为90 .3%、80 .6 %和 96 .8% ,明显高于无淋巴结转移组 5 1.7%、41.3%和 5 5 .1% (P <0 .0 1) ;VEGF、KDR在高分化型胃癌组阳性表达为 40 .0 %、46 .7% ,明显低于低分化、未分化型胃癌组 ,Flt在高分化型胃癌组其阳性表达为 73 .3 % ,低分化、未分化型胃癌组阳性表达为 87.5 % (P >0 .0 5 ) ,VEGF、Flt、KDRmRNA阳性表达与胃癌组织浸润深度无关。结论　VEGF、Flt、KDR与胃癌的一些生物学行为有一定的相关性 ,VEGF、Flt、KDR的高表达有助于胃癌的预后评估更多还原

【Abstract】 Objective Our purpose was to investigate the correlation among the expression of vascular endothelial growth factor(VEGF), its receptor Flt, KDR mRNA and the biological behavior of gastric cancer. Methods 60 cases of gastric cancer patients proved by surgery and pathology were studied with the method of semi quantitative reverse transcription polymerase chain reaction (RT PCR), to determine the expression of VEGF and its receptors Flt, KDR mRNA in gastric cancer tissue. Results The positive percentage of VEGF, Flt, KDR in gastric cancer was 73.3%, 86.7%, 80.0%respectively, in the peri cancerous tissue, the percentage was 43.3%, 33.0% and 30.0% respectively, there were significant difference between the two groups ( P <0.01). For gastric cancer patients with lymphatic metastasis, the positive percentage of VEGF, Flt, KDRmRMA was 90.3%, 80.6%, 96.8% respectively, for patients without lymphatic metastasis, the percentage was 51.7%, 41.3%,55.1%, there were significant difference between the two groups ( P <0.01). The positive percentage of VEGF, KDR in well differentiated gastric cancer was 40.0%, 46.7%, markedly lower than the poor differentiated and undifferentiated gastric cancer groups. The positive expression of Flt in well differentiated gastric cancer group was 73.3%, There was no significant difference compared with poor differentiated and undifferentiated groups (87.5%). The positive expression of VEGF, Flt,KDRmRNA was unrelated with the depth of infiltration. Conclusion There are some correlation between VEGF, Flt, KDRmRNA and the biological behavior of gastric cancer. The high expression of them is of help in evaluating the prognosis of gastric cancer.更多还原