【摘要】 目的研究ＨＳＣ在体外和体内的增殖和凋亡及中药的干预作用。方法体外研究对象为ＨＳＣ系。细胞增殖采用ＭＴＴ比色法，细胞凋亡采用电镜观察、流式细胞仪和ＴＵＮＥＬ法检测。临床研究对象是慢性乙型肝炎患者。结果复方８６１显著抑制体外培养的ＨＳＣ增殖，随着药物剂量的增加和时间的延长，ＨＳＣ的凋亡明显增多，凋亡率增加，呈剂量和时间依赖。 ＴＵＮＥＬ法检测，用 ５ ｍｇ／ｍｌ复方 ８６１作用 ４８ ｈ后， ＨＳＣ凋亡率为 ２５．９％，对照组为９．２％，差异有显著性。在慢性乙型肝炎患者中，肝星状细胞大量活化增生。复方８６１治疗６个月后，活化的星状细胞数量显著减少，可观察到其凋亡。结论活化的肝星状细胞存在凋亡，促进其凋亡在肝纤维化治疗中有重要作用。更多还原

【Abstract】 Objective To investigate the proliferation and apoptosis of the hepatic stellate cell (HSC) in vitro and in vivo and the effects of Chinese herb, compound 861. Methods The in vitro study was carried out on the culture of hepatic stellate cell line. Various concentrations of compound 861 were added and incubated. Cell proliferation was detected with MTT colorimetric assay. Cell apoptosis was detected by electron microscopy, flow cytometry and TUNEL. The subjects of in vivo study were patients with chronic hepatitis B. Results Compound 861 could significantly inhibit HSC proliferation and increase the apoptosis rate of HSC dose-dependently and time-dependently compared with the control group. After compound 861 incubation for 48h, the apoptosis rate of HSC was 25.9% compared with 9.2% in control group(p<0.05). The clinical study elucidated that the HSC was activated and proliferated in patients with chronic hepatitis B. After compound 861 treatment for 6 months, the number of activated HSC decreased and the apoptosis of HSC could be seen in the liver biopsy. Conclusion Apoptosis of activated HSC exists in vitro and in vivo, stimulating its apoptosis may play an important role in the treatment of liver fibrosis.更多还原