【摘要】 目的　观察造血生长因子对受照射小鼠的防治作用。方法　用RSP CM和LP3 CM来源的集落刺激因子治疗受不同剂量60 Coγ射线照射的ICR小鼠。通过观察 30d存活率、死亡动物平均存活天数、保护系数及部分动物外周血白细胞计数、骨髓有核细胞计数和粒单系祖细胞集落培养 ,研究GM CSF对受照射小鼠重建造血的疗效及量效、时效关系。结果　RSP CM能显著提高7 5Gy照射小鼠的存活率 ,而LP3 CM效果不显。提高剂量到 8 0Gy ,只有RSP CM治疗组有效。外周血WBC和BMC计数及祖细胞集落培养也显示治疗组优于预防组。 8 5Gy照射小鼠 30d内全部死亡 ,表明GM CSF对大剂量照射小鼠疗效差。但从保护系数看 ,各实验组均有效 ,且存在量效、时效关系。结论　GM CSF对受照射小鼠的骨髓损伤有促进造血恢复的作用 ,且存在量效、时效关系 ,单纯应用M CSF则无效更多还原

【Abstract】 Objective\ To investigate the radioprotective effects of cytokines on hematopoietic impairment of irradiated mice. Methods\ Using RSP\|CM and LP3\|CM respectively originated GM\|CSF and G\|CSF to treat ICR mice irradiated with different doses of 60 Co γ\|rays.The 30\|day survival rate of mice,the mean survival days of dead mice were determined and the numbers of peripheral white blood cells and BMC of part of the mice were counted.At the same time,GM clonogenic activity of BM was assayed. Results\ RSP\|CM could effectively raise 30\|day survival rate of mice irradiated with 7\^5 Gy.However,LP3\|CM had no obvious effect.Judging from the comparative survival ratio,only the RSP\|CM treated group showed protective effect on the 8\^0 Gy \|irradiated mice.The 8\^5 Gy\|irradiated mice all died within 30 days,indicating that GM\|CSF had weak effect on higher dose\|irradiated mice. Conclusion\ GM\|CSF can stimulate the hematopoietic system of irradiated mice,and has dose\|effect and time\|effect relations.M\|CSF used singly has no obvious effect.\;更多还原