【摘要】 目的　了解达那唑体外对子宫内膜异位症 (内异症 )患者腹腔巨噬细胞释放细胞因子的影响及与胞浆游离钙 ([Ca2 + ]i)浓度的关系。方法　将浓度分别为 10 μg/L、10 0 μg/L、10 0 0 μg/L的达那唑加入 14例内异症不孕患者和 11例盆腔正常的不孕患者 (对照 )的腹腔液中进行培养 ,用双抗体夹心法检测腹腔巨噬细胞培养上清液中肿瘤坏死因子α(TNF α)和白细胞介素 6 (IL 6 )的水平。同时应用Frua 2测定另 10例内异症、8例对照患者的腹腔巨噬细胞 [Ca2 + ]i水平。结果　与对照者相比 ,内异症患者腹腔巨噬细胞释放TNF α和IL 6的能力明显增强 (P <0 .0 1) ,其 [Ca2 + ]i水平也明显升高(P <0 .0 1)。体外达那唑浓度达到 10 0 μg/L时 ,内异症患者腹腔巨噬细胞培养上清液中TNF α和IL 6的水平显著降低 (P <0 .0 1) ,其 [Ca2 + ]i也显著降低 (P <0 .0 1)。结论　达那唑体外能明显抑制内异症患者腹腔巨噬细胞活性。其作用与降低细胞内 [Ca2 + ]i水平有关。更多还原

【Abstract】 Objective To investigate in vitro effect of danazol on cytokine production of macrophages in peritoneal fluid from infertile patients with endometriosis and its relationship with cytosolic free calicum ([Ca 2+ ]i) concentration. Methods Pertitoneal macrophages were obtaind from 14 cases of 24 infertile women with endometriosis (EM group) and 11 cases of 19 infertile women with normal pelvis (control group). Danazol was added to peritoneal macrophages culture reaching final concentrrations of 10 μg/L,100 μg/L,1 000 μg/L respectively. Tumor necrosis factor α(TNF α) and interleukin 6 (IL 6) levels in macrophage supernatant were detected by enzyme linked immunoassay. The [Ca 2+ ]i of cultured peritoneal macrophage and its changes after danazol addition were also studied. Results In EM group TNF α and IL 6 levels in peritoneal macrophage supernatant and [Ca 2+ ]i of macrophages were elevated significantly as compared with those in control group. When danazol was added to the cell culture and reached 100 μg/L of concentration, the levels of TNF α and IL 6 in cultured macrophage supernatant and the [Ca 2+ ]i of macrophages in EM group decreased significantly ( P <0.01). Conclusion It is suggested that danazol may exert its suppressive effect on endometriosis by inhibiting cytokine release of peritoneal macrophages and may related to decline of [Ca 2+ ]i concentration.更多还原