【摘要】 目的　研究胰岛素样生长因子 1(IGF 1)对新生儿免疫功能的影响。方法　利用无血清、无激素培养系统 ,体外培养 10例健康足月顺产儿脐带血单个核细胞 (CBMC)。 10例健康成人外周血单个核细胞 (PBMC)为正常对照。观察IGF 1对CBMC细胞因子产生的影响。结果　与正常组比较 ,植物血凝素 (PHA)诱导的CBMCIL 2、IL 4、干扰素γ(IFN γ)、IL 10和IL 12水平显著低下 (P值均 <0 .0 5 )。单纯IGF 1对CBMC、PBMCIL 2、IL 4、IFN γ和IL 10无明显刺激作用 ,但其可诱导CBMC产生高水平的IL 6及诱导PBMC产生高水平的IL 6和较低水平的IL 12 (P值均 <0 .0 1)。IGF 1可显著提高PHA诱导IL 6和IFN γ产生 (P值均 <0 .0 1) ,并使CBMC产生的IFN γ达到正常成人水平。同时 ,IGF 1可显著提高脂多糖刺激的CBMC产生IL 10 (P值 <0 .0 5 ) ,但尚不能达正常成人水平。相反 ,IGF 1抑制了脂多糖刺激的CBMCIL 12产生 (P值 <0 .0 5 )。结论　IGF 1可促进新生儿T淋巴细胞成熟并调节其免疫功能。更多还原

【Abstract】 Objective To investigate the effects of insulin like growth factor 1 (IGF 1) on neonatal immunity. Methods Cord blood mononuclear cells (CBMC) from 10 normal, full term infants, and 10 adults′ peripheral blood mononuclear cells (PBMC, controls) were involved in this study. CBMC and PBMC were cultured in a serum and hormone free medium. Cytokine activity was measured by ELISA. Results Compared with that of adults, the productions of IL 2, IL 4 and IFN γ in PHA stimulated CBMC were significantly decreased, and the productions of IL 10 and IL 12 in LPS stimulated CBMC were also significantly reduced. IGF 1 alone could not induce IL 2, IL 4, IFN γ and IL 10 productions in CBMC or PBMC. However, it could induce a high level of IL 6 production and a low level of IL 12 production in CBMC or PBMC. IGF 1 significantly increased the productions of both IL 6 and IFN γ in PHA stimulated neonatal MNC. Moreover, it increased neonatal IFN γ production in PHA stimulated CBMC to a level similar to that of adults. In LPS stimulated CBMC, IGF 1 significantly enhanced IL 10 production, but it could not increase neonatal IL 10 production to the level comparable to that of adults. However, IGF 1 suppressed IL 12 production in LPS stimulated CBMC. Conclusion IGF 1 could promote maturation of neonatal T cells.更多还原