【摘要】 目的　以原发性鼻咽癌为研究对象 ,探讨p16抑癌基因 ,myc家族癌基因在鼻咽癌发生、发展及其生物学行为的变化规律。方法　用多重聚合酶链反应 (multiplepolymerasechainreaction ,MPCR)、限制性内切酶PCR、免疫组化及逆转录PCR(reversetranscriptasePCR ,RTPCR)方法 ,检测 6 9例鼻咽癌活检肿瘤组织中 ,P16基因纯合缺失、甲基化、p16基因蛋白表达 (免疫组化 )、c myc基因表达等情况。结果　P16基因纯合缺失 7例 ,甲基化 11例 ,总失活率为 2 6 .1% (18/6 9)。免疫组化检测p16基因蛋白表达阴性 6 0 9% (4 2 /6 9) ;阳性 39.1% (2 7/6 9)。RTPCR ,myc基因检测表达结果 73.9% (5 1/6 9) ,无表达者 2 6 .1% (18/6 9)。结论　研究表明鼻咽癌发生与p16抑癌基因失活、癌基因激活有着密切关系。更多还原

【Abstract】 Objective The alterations of c-myc and p16 genes and their relationship with the development and progression of nasopharyngeal carcinoma were studied. Methods Sixty-nine biopsies of nasopharyngeal carcinoma were examined for the homozygous deletion methylation and reduced expression of p16, and the overexpression of myc family oncogenes using multiple PCR, restriction endonuclease coupled PCR, reverse transcriptase PCR and immunohistochemistry. Results The homozygous deletion and methylation of p16 gene were found in 7 and 11 cases respectively. The total inactivation rate was 26.1% (18/69). The negative expression of p16, as shown by immunohistochemical examination was found in 42 out of 69 cases (60.9%). The overexpression of myc family oncogenes was found in 51 cases (73.9%). Conclusion It suggests that the inactivation of anti-oncogene p16 and the activation of myc family oncogene may play an important role in the development of nasopharyngeal carcinoma.更多还原