【摘要】 目的　探讨Na+通道与鼻息肉形成的关系 ,并进一步探讨白细胞介素 1β(IL 1β)对鼻粘膜上皮细胞Na+通道的影响。方法　采用细胞贴附式膜片钳技术对无血清气 液界面培养的鼻源性鼾症患者下鼻甲和鼻息肉上皮细胞进行研究。结果　①比较与Na+回吸收相关的 3种因素 ,即Na+通道的电导、开放概率 (开放时间 采样时间 )及发生率 (Na+通道的阳性膜片 检测膜片 ) ,发现鼻息肉上皮细胞的电导和发生率均略高于下鼻甲上皮细胞 ,但差异无显著性 (P >0 .0 5 ) ,而鼻息肉上皮细胞的开放概率明显高于下鼻甲上皮细胞 ,差异有显著性 (P <0 .0 1) ;②IL 1β明显增加鼻息肉上皮细胞Na+通道的开放概率及电导 ,与IL 1β加入前相比差异有显著性 (P <0 0 1)。 结论　①Na+通道异常改变与鼻息肉的形成密切相关 ,鼻息肉上皮细胞开放概率增大是导致Na+回吸收增加的重要因素 ;②IL 1β通过延长Na+通道开放时间及增大电导 ,增加细胞膜对Na+的通透能力而促进Na+回吸收 ,在加速鼻息肉形成与发展中可能起到了重要作用。更多还原

【Abstract】 Objective To investigate the relationship between Na+ channels and nasal polyps and to study the effect of IL1β on Na+ channels of epithelial cells of human normal nasal turbinate (NT) and nasal polyps(NP). Methods Serumfree cultured epithelial cells of NT from obstructive sleep apnea syndrome patients and NP were studied on collagen gelcoated membranes at an airliquid interface using the patchclamp technique in the cellattached configuration. Results (1)The three Na+ absorptionrelated factors(the incidence of Na+ channels, the conductance of Na+ channels and the open probability of Na+ channels) were compared in NT cells and NP cells. There was no difference between NT and NP cells in the incidence(P>0.05) and in the conductance (P>0.05). However, the open probability was higher in NP cells compared to NT cells(P<0.01).(2) Application of IL1β increased significantly in the open probability of Na+ channels in NT and in NP cells(P<0.01),the conductance of Na+ channels in NP cells was strongly increased by IL1β(100 ng/ml)(P<0.01). Conclusions (1)The formation of NP is significantly correlated with increased Na+ absorption, the prolonged open time of Na+ channels is one of the most important factors. (2) IL1β may upregulate Na+ permeability by prolonged open time of Na+ channels and increased conductance of Na+ channels, increased absorption of Na+ may lead to the growth and enlargement of NP.更多还原