【摘要】 目的　探讨乳腺癌组织层粘连蛋白 (LN)及其受体 (LN R)和nm2 3蛋白的表达与间质微血管密度及肿瘤转移的关系。方法　应用免疫组织化学LSAB方法检测 73例乳腺癌LN ,LN R和nm2 3蛋白的表达 ,在第Ⅷ因子相关抗原 (FⅧRAg)染色切片上检测其微血管密度 (MVD)。结果　病理分级越高 ,乳腺癌LN表达程度越强 ,即病理Ⅰ级而LN分布Ⅲ级者为 8.3% ,病理和LN分布均Ⅲ级者为 5 8.8% ,两者间差异有极显著意义。LN R表达程度则与转移有关 ,转移组表达程度 (78.6 % )高于非转移组 (2 6 .7% )。LN R的表达程度与乳腺癌间质MVD呈正相关。淋巴结转移率 ,nm2 3表达阳性组与阴性组比较差异有显著性意义。在nm2 3表达抑制时 ,LN R ,MVD增加 ,淋巴结转移明显增加。结论　LN R表达增强 ,MVD增加 ,nm2 3表达抑制 ,可能作为预测肿瘤转移及判断预后的可靠指标更多还原

【Abstract】 Objective To study the relationship between 67 000 laminin, laminin receptor (LN R) and nm23 protein expression, interstitial microvascular density(MVD) and tumor metastasis in breast carcinomas. Methods The expression of laminin (LN), LN R, FⅧRAg and nm23 protein were detected in 73 cases of breast carcinoma with immunohistochemical technique and analyzed. Results A significant difference in LN expression was found in breast carcinoma according to their pothological grade. A positive relation exists between LN R expression and lymph node metastasis. A positive relationship exists between the degree of LN R and MVD expression. A significant difference in lymph node metastasis cases exists between nm23 protein positive expression group and negative expression group. When the expression of nm23 protein was inhibited, the degree of LN R, MVD expression increased and positively related with lymph node metastasis. Conclusion LN R expression enhancement, microvascular density increase and nm23 expression inhibition may be the reliable markers for predicting tumor metastasis and prognosis.更多还原