【摘要】 提取低剂量辐射(LDR)诱导蛋白,对其进行初步检测,观察其生物学活性、对有害理化因子造成细胞损伤的保护作用、对正常人及部分眼疾患者外周血淋巴细胞的刺激作用。小鼠给予 LDR 后,取脾脏细胞超声破碎,再经离心取得 LDR 诱导蛋白提取液,经凝胶过滤、电泳检测新生蛋白及其分子量。用 Lowry’s 法测定新增蛋白含量。用同位素掺入法观察 LDR 诱导蛋白生物学活性及影响其活性的因素、观察其对诸如紫外线(UV)、大剂量 Co γ 射线照 60射、热等有害因素造成细胞损伤的保护作用及其对正常人及部分眼疾患者外周血淋巴细胞转化的刺激作用。制备的 LDR 诱导蛋白提取液经凝胶过滤出现新增蛋白,其分子量为 76.9 KD±～110.0 KD±,产量为 613.33±213.42 μg /3×107个脾细胞。经 5～15 cGy LDR后 2～16 h 都可从小鼠脾脏细胞中提取得到 LDR 诱导蛋白并具有生物学活性,它可刺激离体小鼠脾脏细胞的转化,对某些有害理化因子造成的细胞损伤有明显的保护作用并具有刺激正常人及部分眼疾患者外周血 T,B 淋巴细胞转化的免疫增强作用。更多还原

【Abstract】 The protective effects of low dose radiation (LDR) induced protein on cellular impairments caused by some harmful chemical and physical factors were studied. Male Kunming mice were irradiated with LDR, then the spleen cells of the mice were broken with ultrasonic energy and then ultracentrifugalized. The supernatant solution contained with LDR induced protein. The newly emerging protein was detected by gel filtration and its molecular weight was determined by gel electrophoresis. The content of newly emerging protein (LDR induced protein) was determined by Lowry’s method. The method of isotope incorporation was used to observe the biological activity and its influence factors, the protective effects of LDR induced protein on the cells impaired by irradiating with ultraviolet (UV), high doses of Co γ-rays and exposed to heat respectively, and the stimulative effects of 60 LDR induced protein on human peripheral blood lymphocytes. Newly emerging protein has been observed in the experiment. The molecular weight of the protein is in the region 76.9 KD±～110.0 KD±, the yield of the protein was 613.33±213.42 μg per 3×107 spleen cells. DPM values (isotope were incorporated) of normal and injured mice spleen cells increased significantly after stimulating with the solution contained LDR induced protein. It is concluded that LDR induced protein could be obtained from mice spleen cells exposed to 5～15 cGy radiation for 2～16 h. The protein had biological activity and was able to stimulate the transformation of the spleen cells in vitro. It had obvious protective effects on some impaired cells caused by high dose radiation, UV radiation, heat and so on. It also had stimulative effects on the transformation of peripheral blood T and B lymphocytes of healthy individual and patients with eye diseases. It indicates that LDR induced protein increased immune function of human.更多还原