【摘要】 目的　研究 2 3 羟基桦木酸对体内、外B16黑色素瘤细胞生长的抑制及诱导凋亡作用。方法　应用MTT分析 ,电镜观察 ,流式细胞仪 ,肿瘤体积大小测定等方法 ,对 2 3 羟基桦木酸诱导黑色素瘤细胞进行检测和观察。结果　2 3 羟基桦木酸体外 3 0～ 80 μg/ml处理 ,体内 3 0 0mg/kg～ 6 0 0mg/kg灌胃时 ,对B16细胞有显著的生长抑制作用 ,有一定的剂量依赖关系并出现大量的凋亡细胞 ,同时体内肿瘤细胞被阻滞在G0 ～G1期 ,DNA合成受阻 ,肿瘤体积缩小。结论　 2 3 羟基桦木酸对体内外黑色素瘤细胞增殖有强烈的抑制作用 ,诱导肿瘤细胞死亡的主要途径是凋亡。更多还原

【Abstract】 ? Objective To study 23 hydroxyl betulinic acid induced apoptosis and growth inhibition of B16 melanoma cell in vivo and in vitro.Methods MTT assay,electronic microscopy,flow cytometry and tumor volume assay were used to examine the effect of 23 hydroxyl betulinic acid on B16 melanoma cell line.Results 30～80μg/ml 23 hydroxyl betulinic acid in vitro and 300mg/kg～600mg/kg P.O.in vitro markedly inhibited the growth of B16 cells at a dose dependent manner with a great number of apoptotic cells seen.Tumor size decreased markedly.Flow cytometry showed cell accumulation at G 0～G 1 stage and DNA synthesis retardation.Conclusions 23 hydroxyl betulinic acid potently inhibited the growth of B16 cell that functioned by induction of apoptosis. 〔更多还原