【摘要】 目的:考察阿托品输液的稳定性,探讨阿托品输液法抢救有机磷中毒患者的可行性。方法:建立RPHPLC法[色谱柱为NovaPakC184μm,3.9×150mm,流动相为甲醇/水(28∶72)]加入三乙胺0.12%,并用冰醋酸调pH5.0,检测波长210nm,流速0.8ml·min-1,柱温40℃,氢溴酸山莨菪碱做内标,检测临床应用条件下阿托品含量变化。另测定pH、不溶性微粒及外观变化。结果:在临床应用条件下阿托品含量、pH、外观24h稳定。经临床应用,阿托品输液法抢救有机磷中毒29例效果满意。结论:阿托品输液法抢救有机磷农药中毒是切实可行的。这对于中度、重度中毒患者减少被反复穿刺的痛苦、减少护理工作量具有重要的临床意义更多还原

【Abstract】 OBJECTIVE:This paper was to observe the stability of atropine sulfate in normal infusion and to serve basical data for clinical application.METHODS:Atropine sulfate 0.1 or 1.0 mg·ml -1 was combined with infusion.The concentration of atropine was determined by an established RP HPLC.A Nova Pak C 18 column ( 3.9 ×150 mm) and 28% methanol in water and 0.12% triethylamine with acetic acid adjusting pH 5.0 as the mobile phase and the flow rate of 0.8 ml·min -1 and column temp.Of 40℃ were adopted and the detection wavelength of 210nm was specified.Anisodamini hydrobromidium used as an internal standard.The solution were visually assessed for color and clarity,and particulate content was measured with a particle counter,and pH was measured.RESULTS:All solutions containing atropine retained more than 90% of their initial levels.pH and appearance had no significant change.The consequence of clinical application is satisfying in 29 patients of organic phosporous poisoning.CONCLUSIONS:Atropine sulfate was stability in normal infusion for 24h and used by infusion was feasible.It has clinical implication to the medial or severe organic phosporous poisoner.更多还原