节点文献

甘蓝型油菜二体附加系92Ⅰ 1096附加染色体的遗传研究

Cytological studies on alien addition chromosome of disomic addition line 92Ⅰ1096 in Brassica napus L.

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 戚存扣高冠军浦惠明傅寿仲仲裕泉

【Author】 QI Cun kou 1, GAO Guan jun 1, PU Hui ming 1, FU Shou zhong 1, ZHONG Yu quian 2 (1. Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China; 2. Institute of Bio Genetics and Physiology,Jiangsu Academy of Agr

【机构】 江苏省农科院经济作物研究所!南京210014江苏省农业科学院遗传生理研究所!南京210014

【摘要】 应用细胞学方法研究了甘蓝型油菜二体附加系 92 1 0 96( Brassica napus-carinata2 n=4 0 )和甘蓝型油菜整倍体品种宁油 1 0号 ( B.napus L.2 n=3 8)及其杂种的体细胞染色体数及花粉母细胞减数分裂过程中的染色体配对行为。结果 ,甘蓝型油菜二体附加系 92 1 0 96的体细胞染色体数稳定在 2 n=4 0条。它与宁油1 0号间的正反交杂种体细胞染色体数为 2 n=3 9条。表明二体附加系 92 1 0 96的两条外源的同源附加染色体通过雌雄配子的传递机会相等。花粉母细胞减数分裂中期 ( MI) ,杂种的 3 9条染色体配对成 1 9 +1 I,少数细胞出现了多价体 ,但在观察的所有花粉母细胞中均携有一个单价体。 92 1 0 96花粉母细胞减数分裂晚后期( AI) ,二分体细胞的染色体构型为 2 0 +2 0的细胞占 93 .54%。表明该二体附加系的附加染色体能在杂种后代中稳定传递 ,不易因遗传排斥而丢失 ,可用来进行有关农艺性状的基因定位研究

【Abstract】 Chromosome pairing behavior in pollen mother cells meiosis and chromosome number in somatic cells were observed on a disomic addition line, 92Ⅰ1096 ( Brassica napus carinata , 2n=40), and a euploid variety of Ningyou 10 ( Brassica napus , 2n=38) and their hybrid. Results showed that the chromosome number in somatic cells of disomic addition line, 92Ⅰ1096, was 2n=40. And the chromosome number in somatic cells of reciprocal hybrid between 92Ⅰ1096 and Ningyou 10 were 2n=39. It is indicating that the two homologous alien chromosomes in the disomic addition line 92Ⅰ1096 transmitted to next generation by male or female gametes with an equal frequency. At metaphase I of PMCs first meiosis, the chromosome configuration of the hybrid was 19Ⅱ+1Ⅰ. All the PMCs observed carried one univalent, although one multivalent was found in some PMCs. At late anaphase, 93.6% of the dyad observed showed a chromosome configuration of 20+19. A conclusion could be made that the alien addition chromosomes in the disomic addition line can steadily pass on to its offspring, which can be used for gene mapping.

【关键词】 甘蓝型油菜二体附加系遗传
【Key words】 Disomic addition lineCytogeneticsBrassica napus
【基金】 国家自然科学基金!资助项目 ( 3 95 70 45 8)
  • 【文献出处】 中国油料作物学报 ,CHINESE JOURNAL OF OIL CROP SCIEVES , 编辑部邮箱 ,2000年01期
  • 【分类号】S565.403.51
  • 【被引频次】15
  • 【下载频次】77
节点文献中: 

本文链接的文献网络图示:

本文的引文网络