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样品库PCR法检测大学生沙眼衣原体感染状况的研究

Pooling of Urine Specimens for PCR Method to Detect Chlamydia Trachomatis Infection among University Students

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【作者】 高翔董兆文谭永平乌正赉

【Author】 GAO Xinang, DONG Zhao - wen , TAN Yong - ping , et al. Institute of Basic Medical Sciences, Peking Union Medical College and Chinese Academy of Medical Sciences , Beijing 100005 , China

【机构】 中国协和医科大学基础医学院流行病教研室国家计划生育委员会科学技术研究所北京师范大学生命科学学院中国协和医科大学基础医学院流行病教研室 北京 100005北京 100005

【摘要】 目的 了解大学中沙眼衣原体((Chlamydia Trachomatis,CT)感染状况,探索有效的CT筛查方法,为制定中国青少年中的STDs的预防措施提供科学依据。方法 于1999年在北京某综合性大学学生中,随机抽取2050人,进行间卷调查并调查人群中的Cr感染率。结果 在1935份有效尿样中,共检出阳性3例,检出率为1.6%。结论在低CT感染率人群中进行筛查,样品库(Pooling)法为一行之有效的筛查方法,并提示在大学生中,生殖健康之重点应为安全性知识、性道德的宣传。

【Abstract】 Objective To estimate the prevalence of Chlamydia Trachomatis ( CT) infection in university students and explore effective way to screen CT infection. Methods and Results 2050 university students in Beijing were randomly selected in June 1999, and the first catch urine (FCU) samples were collected from each of them to detect CT infection. 1935 effective FCU samples were returned. CT DNA was extracted and detected Using Pooling of urine specimens by PCR and ELJSA , in which 3 were positive. Conclusions Results of this study show that pooling of FCU samples for PCR testing is a recommendable measure in population of low infectious rate, and reproductive health education targeting the college students should focus on the mobilization of sex morality and knowledge of safe sex.

【基金】 中国-欧盟性病艾滋病防治培训项目
  • 【文献出处】 中国预防医学杂志 ,China Preventive Medicine , 编辑部邮箱 ,2000年01期
  • 【分类号】R181.3
  • 【被引频次】1
  • 【下载频次】31
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