【摘要】 为探讨肿瘤坏死因子 - α(TNF- α)诱导 K562 凋亡的作用 ,应用细胞形态学观察、DNA琼脂糖凝胶电泳 ,原位末端标记法检测 K562 、TNF-α诱导的 K562 。结果　表明 ,细胞形态学观察 K562 自然凋亡率 (2 .9± 0 .7) % ,TNF- α5 0 ng/ml、1 0 0 ng/ml诱导的 K562 凋亡率分别为 (1 6 .5± 4.1 ) % ,(2 3.9± 4.8) %。 (F=89.9,P<0 .0 1 )。 DNA琼脂糖凝胶电泳 TNF- α1 0 0 ng/ml诱导的 K562 DNA电泳呈梯状条带。原位末端标记法K562 自然凋亡率 (3.5± 1 .0 ) % ,TNF- α5 0 ng/ml、1 0 0 ng/ml诱导的 K562 凋亡率分别是(2 1 .4± 4.5 ) %、(2 7.4± 4.9) %。 (F=1 0 4.9,P<0 .0 1 )。结论　 TNF-α 5 0 ng/ml、1 0 0 ng/ml能诱导 K562 凋亡更多还原

【Abstract】 To explore Tumor necrosis Factor α (TNF α) induced K 562 apoptosis. Apoptosis was detected by TDT end labelling,wing cell morphorlogy and DNA agarose gel electrophoresis technigues. The ratio of K 562 apoptosis was determined in K 562 、K 562 induced by TNF α. Results showed with cell morphorlogy,K 562 natural apoptotic rato was (2.9±0.7%),the ratio of K 562 apoptosis induced by TNF α(50ng/ml、100ng/ml) was (16.5±4 1%),(23 9±4 8%)(F=89.9,P<0 01);with TUNEL,K 562 natural apoptotic ratio was (3.51.0)%,the ratio of K 562 apoptosis induced by TNF α(50ng/ml、100ng/ml) was (21.4±4.5)%,(27 4±4.9)% respectively (F=104.9,P<0.01) Thesegose, TNF α(50ng/ml、100ng/ml) could induce K 562 apoptosis.更多还原