【摘要】 根据 ＵＳＤＡ（美国农业部）标准攻毒株 ＩＬＴＶ ｇＩ－ｇＥ基因的序列，在 ＩＬＴＶ ｇＩ基因、ｇＥ基因及 ｇＩ－ｇＥ基因连接部的上下游分别设计了一对引物。以 ＳＤＳ－蛋白酶 Ｋ法提取的 ８株 ＩＬＴＶ ＤＮＡ为模板，分别用这 ３对引物进行 ＰＣＲ扩增，均获得了与预期片段大小一致的ＤＮＡ片段。然后，用４种限制性内切酶（Ａｖａ Ⅱ、ＨｉｎｆⅠ、ＤｄｅⅠ和ＨａｅⅢ）对所有ＰＣＲ产物进行ＲＦＬＰ分析。结果发现， ＨｉｎｆⅠ和 ＤｄｅｌⅠ可以分别将 ８株 ＩＬＴＶ ｇＥ基因的酶切图谱分为两类， ＨａｅⅢ可将 ８株 ＩＬＴＶ的ｇＩ－ｇＥ基因连接部的酶切图谱分为两类。这表明不同的ＩＬＴＶ毒株的ｇＥ基因变异较大，而ｇＩ基因则相对比较保守。更多还原

【Abstract】 Three pairs of primers were designed based on the sequence of gI, gE, and gI-gE junction region of ILTV USDA challenge strain. The DNAs were extracted from eight ILTV strains and subjected to amplification using the primers by PCR. Then the amplified fragments were digested by four kinds of restriction enzymes, Ava .Hinf .Dde Hae , respectively.The results showed that PCR products amplified from all eight ILTV generated the same pattern after digestion with each of four enzymes, while eight ILTV could be divided into ho groups after digestion of PCR products from gE with Hinf .and Dde . When analyzing PCR products from gI-gE junction region,only Hae created two different patterns among the eight viruse, and other thee enzymes gave the same patterns to all viruses.These results proveded some ba- sis for further study on correlation between variation of virulence-related genes and virulence of ILTV.更多还原