【摘要】 参考Genebank发表的传染性支气管炎病毒 (IBV)S1纤突蛋白基因序列 ,自行设计合成了 1对引物 ,对IBV青岛腺胃分离株 (SD/97/0 2 )RNA进行RT PCR扩增。产物经琼脂糖凝胶电泳分析 ,呈现 1条 16 5 7bp的条带 ,将其克隆入pMD18 T载体中 ,并进行序列测定 ,证实为S1基因。将此重组质粒命名为pMDQXS1。更多还原

【Abstract】 A pair of primers were designed and synthesized according to the S1 spike protein gene sequence of IBV reported by Genebank. Then the viral RNA of QXIBV (SD/97/02) was amplified by a reverse transcription polymerase chain reaction(RT PCR).The amplified product was analyzed by agarose gel electrophoresis, all of which showed a fragment of 1657 bp as expected.The RT PCR product was cloned into the pMD18 T vector ,and then the recombinant plasmid was sequenced, which was proved to be the S1 spike gene of IBV. The recombinant plasmid was designated as pMDQXS1.更多还原