【摘要】 目的 :研究中药丹参 (SM )对心肌低氧 /复氧损伤的保护作用。方法 :运用31P NMR技术对离体灌流大鼠心脏的高能磷酸化合物含量及细胞内的 pH值 (pHi)进行动态跟踪。结果 :丹参注射液能明显减轻低氧期间心肌高能磷酸合物含量的下降 ,促使复氧期间PCr、ATP相对含量的恢复 ,减少低氧及复氧阶段心肌 pHi的下降。 结论 :丹参能改善低氧及复氧期间心肌能量代谢水平 ,减轻心肌低氧 /复氧损伤 ,并能显著改善细胞内酸碱平衡状况更多还原

【Abstract】 Aim and Methods: To assess the myocardial effects of Salvia Miltiorrhiza (SM) injection against hypoxia reoxygenation injury, 31 P NMR was used to trace the time courses of high energy phosphates (HEP) content and intracellular pH (pHi) of the isolated perfused rat hearts under hypoxia (30 min) and subsequent reoxygenation (40 min). Results: It was discovered that SM significantly preven ted the decrease in the myocardial HEP content during hypoxia, enhanced the recovery of myocardial phosphocreatine (PCr),adenosine triphosphate (ATP) and the PCr/Pi (inorganic phosphate) ratios during reoxygenation, and lightened the decrease of the myocardial pHi value caused by hypoxia. Conclusion: SM improves the myocardial energy metabolism level during prolonged hypoxia and subsequent reoxygenation and protects myocardium against hypoxia/reoxygenation injury. SM significantly attenuates acidosis during hypoxia and prevents the appearance of very acidic areas of the myocardium after reoxygenation.更多还原