【摘要】 目的　研究 p16抑癌基因与卵巢上皮性浆液性肿瘤发生与发展的关系。 方法　应用原位杂交和免疫组化法 ,对 47份卵巢上皮性浆液性肿瘤组织和 8份正常卵巢组织 p16mRNA与 p16蛋白进行检测。 结果　p16mRNA与 p16蛋白阳性表达皆主要位于胞浆内 ,呈红色颗粒状 ,阳性表达细胞多呈灶状分布。p16mRNA与 p16蛋白在卵巢浆液性癌中检出率 (4 8 5 %、42 4% )皆低于良性浆液性瘤 (均 90 0 % )及正常卵巢组织 (均 10 0 0 % ) ,均P<0 0 5。p16mRNA、p16蛋白低表达与卵巢浆液性癌的恶性程度高、肿瘤分化差、癌瘤播散及淋巴结转移有关。一致性检验显示 p16基因的原位杂交和免疫组化检测结果关系密切 (P <0 0 1)。结论　p16作为一种新型抑癌基因 ,其mRNA或蛋白低表达与卵巢浆液性癌发生、发展密切相关。更多还原

【Abstract】 Objective To study the relationship between tumor suppressor gene p16 and the generation and development of ovarian epithelial serous tumors.Methods By use of immunohistochemistry and in situ hybridization,the tumor suppressor gene p16(p16 mRNA,p16 protein)were detected in 47 samples of ovarian serous tumors and 8 samples of normal ovarian tissues.Results p16 mRNA and p16 protein positive red granules were mainly intracytoplasmic.The positive cells were chiefly distributed in focuses.The positive expression rate of p16 mRNA and p16 protein in ovarian epithelial serous carcinoma were 48\^5% and 42\^4%,significantly lower than that in benign(90%)and normal ovarian tissues(100%),P<0\^05.Low expression of p16 mRNA,p16 protein were significantly associated with higher degree of ovarian serous carcinoma,poorl differentiation,invasiveness and implantation of carcinoma and lymph nodes metastasis(P<0\^05 or P<0\^01).The expression of p16 at protein level was significantly correlated with that at mRNA level.Conclusion The results suggest that low expression of p16 is well related with the generation and development of ovarian serous carcinoma.更多还原