【摘要】 目的:建立快速测定糖尿病大鼠Alc型糖基化血红蛋白的FPLC方法。方法:大鼠血样经洗涤,离心,甲苯破膜,稀释后;用丙二酸钠溶液(A液)和丙二酸钠-氯化钾溶液(B液)为基本流动相,采用梯度洗脱程序在Mono S HR 5/5阳离子交换柱上进行分离测定,检测波长为415nm。结果:在所给色谱条件下,大鼠Alc型糖基化血红蛋白能很好地从总血红蛋白中分离出来。糖尿病大鼠中HbAlc含量为3.6％±0.6％(n=7)显著高于正常大鼠的1.4％±0.4％(n=7),P<0.01。测定一个样品所需的总时间约为27min。结论:本方法简便,快速,准确,重现性好,可用于大鼠糖尿病模型中糖基化血红蛋白含量的研究和糖尿病治疗药物的筛选。更多还原

【Abstract】 To develop a fast protein liquid chromatography (FPLC) method for the assay of Ale-type glycosylated hemoglobin in diabetic rats. METHODS: Venous blood was collected from rats. After the erythrocytes were washed and sedimented, the hemolysate was prepared and diluted with isotonic saline as a sample for the assay. Samples were then separated on Mono S HR 5/5 cation exchange column by a lithium chloride gradient elution system. The Alc-type glycosylated hemoglobin was monitored by measuring the absorbance at 415 nm. RESULTS: The Ale-type glycosylated hemoglobin was well separated from total hemoglobin. The average HbAlc amount in diabetic rats was determined to be (3.6 %±0.6 %, n = 7), while the corresponding figure in normal rats was (1.4 %±0.4 %, n = 7, P < 0.01). CONCLUSION: This FPLC method is easy, rapid and reproducible, and can be used for an assay of diabetic rat HbAlc to evaluate and screen new drugs for diabetes mellitus therapy.更多还原