【摘要】 目的:建立一种简便灵敏的气相质谱法用于测定丙卡特罗的血药浓度。方法:采用电子轰击离子化气相质谱法,以丙咪嗪做内标,样品采用液-液萃取、衍生化处理,分离柱为毛细管柱,进样器和接口温度分别为280℃和250℃,载气（氦气）流速为0.8 mL·min^-1,进样口选择脉冲不分流模式,离子源和四极杆的温度分别为230℃和150℃。结果:测定方法的检测限为5 ng·L^-1;线性范围为10-10000 ng·L^-1;日内（n=5）和日间（n=5）变异系数均小于10％,平均回收率为99.1％±1.3％。结论:本方法灵敏、简便,可用于丙卡特罗的药代动力学研究。更多还原

【Abstract】 AIM: To improve a gas chromatography/electron impact ionization mass spectrometry (GC/MS) method for determining the concentration of procaterol in human plasma. METHODS: GC/MS was developed with capillary column. Samples were extracted by liquid phase before derivated. Imipramine was used as an internal standard. The injector and GC/MS interface temperatures were set at 280 ℃ and 250 ℃, respectively. The carrier gas (helium) was 0.8 mL·min-1, and injections were made in the pulse-splitless mode. The MS source and MS Quad temperature were 230 ℃ and 150℃, respectively. RESULTS: The detection limit of plasma procaterol was 5 ng·L-1. The assay was linear over the range of 10-10000 ng · L-1 with correlation coefficient of 0. 9987. The coefficients of variation were less than 10 % for procaterol detection at high, medium and low concentration levels (n = 5). The average recovery of the assay was 99.1 % ±1. 3 % . CONCLUSION: This assay was sensitive, precise, and accurate for evaluating the clinical pharmacokinetics of procaterol.更多还原