【摘要】 目的:研究THPB对大鼠肝、脑脂质过氧化（LPO）和氧自由基的影响。方法:体外测定大鼠肝、脑匀浆LPO和Fe²⁺-Vit C诱导肝、脑线粒体LPO产生的MDA,Fenton反应产生OH和邻苯三酚氧化产生O₂^-。结果:（1）THPB减少肝匀浆和线粒体MDA,其中l-THPB-18和SPD的IC₅₀为3.1和12.7μmol·L^-1。SPD减少脑匀浆和脑线粒体MDA,IC₅₀分别为102和35.0μmol·L^-1。（2）THPB能清除OH^-,其中I-THPB-18和SPD的IC₅₀为0.21和3.8μmol·L^-1;但对O₂无抑制作用。结论:THPB能减少MDA和清除OH^-,其中THPB-18作用最强。更多还原

【Abstract】 AIM: To study the effects of tetrahydroprotoberberines (THPB) on rat liver and brain lipid peroxidation (LPO) and oxygen free radicals generation. METHODS: The malondialdehyde (MDA) levels in rat brain and liver ho-mogenates, induction of MDA by Fe2+-Vit C in mitochondria, OH generation by Fenton reaction, and O2 generation by pyrogallol oxidation were observed in vitro. RESULTS: (1) THPB lowered the MDA contents in the liver homogenate and mitochondria, and the IC50 values of l-THPB-18 and l-stepholidine (SPD) in the liver mitochondria were 3.1 and 12.7 μmol·L-1 respectively. SPD decreased the MDA contents in the brain homogenate and mitochondria with IC50 values of 102 and 35.0μmol·L-1 respectively. (2) THPB scavenged OH, and the IC50 values of l-THPB-18 and SPD were 0.21 and 3.8μmol·L-1 respectively, but no effect on O2 was observed. CONCLUSION: THPB could reduce the MDA contents and scavenge OH and THPB-18 was the most potent amongst them.更多还原