【摘要】 目的:研究异型南五味子丁素（HD）和戈米辛J（GJ）对豚鼠心肌L-型钙离子通道的作用。方法:全细胞膜片箝记录。结果:异型南五味子丁素1,10μmol/L及戈米辛J 10μmol/L可抑制L-型Ca²⁺电流。HD和GJ对钙电流稳态激活都无影响,但它们可改变钙电流的稳态失活,提示两种药物作用于L-型钙通道的失活态。结论:HD和GJ对豚鼠心室肌细胞L-型钙离子通道有阻断作用。更多还原

【Abstract】 AIM: To study the effects of heteroclitin D (HD) and gomisin J (GJ), two lignans from Kadsura medicinal plants, on L-type calcium channels in ventricular cells of guinea pig. METHODS: The calcium currents were measured by whole-cell patch-clamp recording technique. RESULTS: HD 1 and 10 μmol/L decreased the L-type calcium current from (770 ± 155) to (482 ± 104) and (384 ±85) pA, respectively. GJ 10 μmol/L inhibited calcium current from (822 ± 169) to (436 ± 143) pA. Neither HD nor GJ affected the steady-state activation curve. But they had impact on steady-state inactivation curve. HD 10 μmol/L changed the half inactivation voltage ( V0.5) from -22.7 to -40.9 mV, and slope factor (k) from 10.2 to 20r6 (n =4 cells from 3 guinea pigs, P <0:05). GJ 10 μmol/L changed the V0.5 from -17.7 to -33.3 mV, and K from 15.9 to 27.8 (n = 5 cells from 3 guinea pigs, P < 0.05). CONCLUSION: HD and GJ inhibited L-type calcium channels.更多还原