【摘要】 选择 42 4例有偿献血员用ELISA检测抗 -HCV ,对抗 -HCV阳性血清用ELISA进行血清学分型 ,对己确定血清型者采用RT—PCR作基因分型。结果 42 4例献血员中 ,HCV感染率为 2 1 70 % ( 92 / 4 2 4) ,献全血并血浆者的HCV感染率( 45 14% )显著高于只献全血者 ( 6 94% ) ;92例抗—HCV阳性者中 ,血清学分型率为 34 78% ( 32份 ) ,其中血清学Ⅰ型 30例( 93 75 % )、Ⅱ型 2例 ( 6 2 5 % ) :该 32例血清中有 30例可用基因分型 ,其中基因Ⅱ型 2 6例 ( 86 6 7% )、Ⅲ型 3例 ( 10 % )、Ⅱ /Ⅲ型混合感染 1例 ( 3 33% ) ,两种分型方法的一致率为93 33 % ( 2 8/ 30 )。表明山东省部分地区献血人群的HCV感染率较高 ;献血浆是该献血人群HCV感染的危险因素 ;其HCV感染以血清学I型和基因Ⅱ型为主更多还原

【Abstract】 To investigate the distribution of HCV infection and subtypes among blood donors in some areas of Shandong Province. Method Serum samples were collected from 424 paid blood donors. Anti-HCV was detected by enzyme-linked immunosorbent assay (ELISA).Serotypes of anti-HCV positive sera were identified by ELISA and genotypes were determined by RT-PCR.Results The HCV prevalence of 424 blood donors was 21 .70% (92/424). It was significantly higher in the donors donating blood and plasma (45. 14%) than in the donors with only blood donation (6.94%). Of 92 anti-HCV positive sera detected, 32 (34.78%) were typed by ELISA. 30 (93.75%) were serotype I, and 2 (6.25%) serotype Ⅱ. Of these 32 sera, 30 were determined by RT-PCR genotype method. HCV genotypes Ⅱ,Ⅲ and Ⅱ/Ⅲ were 26 (87.67%),3(10%), and Ⅰ(3.33%), respectively. The coincidence rate of the two methods was 93.33% (28/30).Conclusion The prevalence of HCV among blood donors in some areas of Shandong Province was relatively high. Plasma-donation was a risk factor of HCV infection in blood donors.The prevalent subtypes of HCV were serotype I and genotype Ⅱ.更多还原