【摘要】 目的 :研究高强度聚焦 (HIFU)超声对人膀胱癌细胞株 T2 4周期分布的影响及联合甲氨喋呤(MTX)的意义。方法 :流式细胞仪 (FCM)检测经 HIFU(4 5 0 w/cm2× 6′)作用后残活细胞的周期分布 ;生长曲线观察 HIFU联合 MTX对 T2 4细胞生长增殖的影响。结果 :经 HIFU照射后 3h残活细胞 G0 % + G1 %下降 ,S%及 G2 %+ M%升高 (P<0 .0 5 ) ,2 4h后 G0 % + G1 %进一步下降而 S%进一步升高 (P<0 .0 5 ) ,12 0 h后恢复正常 (P>0 .0 5 ) ;HIFU联合 MTX对 T2 4细胞生长抑制作用明显强于单用 HIFU、单用 MTX。结论 :在体外以空化、机械效应为主的 HIFU可使 T2 4细胞周期分布发生可复性改变 ,联合 MTX作用具有协同效应更多还原

【Abstract】 Objective:To study the effects of high intensity focused ultrasound(HIFU)on cell cycle distribution of human bladder cancer cell and the imports of combination with Methotrexate(MTX).Methods:Using flow cytometry(FCM)examined cell cycle distribution of survival T 24 cells which irradiated by HIFU(450w/cm 2×6′). Observed cell growth curves of T 24 cells which acted by HIFU and MTX. Results:Through irradiation of HIFU, G 0%+G 1% of the survived cells decreased while S% and G 2%+M% increased 3h later( P <0 05). S% further increased while G 0%+G 1% and G 2%+M% decreased 24h later( P <0 05). Significant difference was not found between test group and control group 120h later(P<0 05). HIFU(450w/cm 2×6′)combined with MTX can halt T 24 cell growth stronger than HIFU alone or MTX alone.Conclusion:HIFU could change the T 24 cell cycle distribution temporarily and combined MTX has sysnergistic inhibition effect.更多还原